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Gene expression of anabolic and catabolic components and microRNAs in skeletal muscle of pacu (Piaractus mesopotamicus)during fasting and refeeding conditions

Grant number: 13/25915-1
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): April 01, 2014
Effective date (End): March 31, 2017
Field of knowledge:Agronomical Sciences - Fishery Resources and Fishery Engineering
Principal Investigator:Maeli Dal Pai
Grantee:Tassiana Gutierrez de Paula
Home Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Skeletal muscle in fish comprises 40-60% of total body and the growth occurs by fibers hypertrophy and/or hyperplasia, mechanisms that vary among the species, stage of development and nutritional status. The growth, development and muscle phenotype are controlled by pathways responsible for muscle-specific genes activation or inhibition, events that can regulate muscle catabolic and anabolic state. Based on this information, studies involving the molecular mechanisms controlling the muscle mass are fundamental to better understand the changes in anabolic and catabolic homeostasis that can occur during fasting and refeeding conditions. In this study we will evaluate the skeletal muscle of pacu (300g) during periods of fasting (10 days) and refeeding (05 days) in the following parameters: (a) muscle fibers morphology and morphometry, (b) gene expression of the components of the pathways related to catabolism (Foxo - MuRF - MAFbx) and anabolism (IGF - PI3K - AKT - mTOR) and the MyoD family of transcription factors (MyoD , Myf5 , myogenin and MRF4), (c) the expression profile of muscle specific microRNAs (miR - 1, miR - 133a, miR - 133b, miR - 206 and miR -499) and yours target genes highly expressed in vivo and (d) the differentially expressed microRNAs and target genes after silencing in primary culture of myoblasts. The fish will be rearing in tanks of water (0.5m³) with distinct recirculation systems. Samples of red and white muscle (n = 10) will be removed every 2 days of fasting and every 6 hours of refeeding periods. In the experiments in vivo and in vitro, the gene expression and microRNAs will be detected by quantitative real-time polymerase Chain Reaction (RT-qPCR).The silencing of microRNAs more expressed in vivo in red and white muscles during fasting will be realized, in vitro, using oligonucleotide antisense inhibitors.The data will be submitted to appropriate statistical analysis after normality and homoscedasticity tests.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE PAULA, TASSIANA GUTIERREZ; THOMAZINI ZANELLA, BRUNA TEREZA; DE ALMEIDA FANTINATTI, BRUNO EVARISTO; DE MORAES, LEONARDO NAZARIO; DA SILVA DURAN, BRUNO OLIVEIRA; DE OLIVEIRA, CAROLINE BREDARIOL; SIMOES SALOMAO, RONDINELLE ARTUR; DA SILVA, RAFAELA NUNES; PADOVANI, CARLOS ROBERTO; DOS SANTOS, VANDER BRUNO; MARECO, EDSON ASSUNCAO; CARVALHO, ROBSON FRANCISCO; DAL-PAI-SILVA, MAELI. Food restriction increase the expression of mTORC1 complex genes in the skeletal muscle of juvenile pacu (Piaractus mesopotamicus). PLoS One, v. 12, n. 5 MAY 15 2017. Web of Science Citations: 8.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
PAULA, Tassiana Gutierrez de. Expressão de genes das vias anabólicas e catabólicas e de miRNas no músculo esquelético do Piaractus mesopotamicus durante período de jejum e realimentação. 2017. Doctoral Thesis - Universidade Estadual Paulista "Júlio de Mesquita Filho" Instituto de Biociências (Campus de Botucatu)..

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