Local and systemic inflammatory profile of rats wistar with multiple endodontics infections

• Grant number: 13/23358-8
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: April 01, 2014
• End date: February 29, 2016
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Luciano Tavares Angelo Cintra
• Grantee: Renata Oliveira Samuel
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

Based on findings of the current literature, of which some results were obtained by our research group, the aim of this work will contribute to understanding of the mechanisms that involve the relationship between infections of endodontic origin and state of bodily homeostasis. Thus, we propose to investigate the profile of local inflammatory induced apical periodontitis and correlate with changes in the profile systemic model with a low wall or multiple infections of endodontic origin. Will be used 30 Wistar rats divided into three groups of 10 animals each: G1 - healthy mice; G2 - mice with endodontic infection alone; G3 mice with multiple endodontic infections. After 30 days, blood will be collected by cardiac puncture for complete blood count (RBCs, packed cell volume, hemoglobin, mean corpuscular volume, mean corpuscular hemoglobin concentration, leukocytes, neutrophil, lymphocyte, monocyte, eosinophil, basophil and platelets), serum quantification of proinflammatory cytokines (IL-17, IL-23, IL-6, TNF-±, IFN-³) by ELISA and nitric oxide. After blood collection, the animals will be killed, the jaws will be processed for light microscopy in HE staining and immunohistochemical staining of inflammatory mediators above. The results of the different analyzes and the correlation between the local and systemic findings will be analyzed by statistical tests specific to each case ( p < 0,05 ).

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SAMUEL, RENATA OLIVEIRA; ERVOLINO, EDILSON; DE AZEVEDO QUEIROZ, INDIA OLINTA; AZUMA, MARIANE MAFFEI; FERREIRA, GABRIELA TIAGO; ANGELO CINTRA, LUCIANO TAVARES. Th1/Th2/Th17/Treg Balance in Apical Periodontitis of Normoglycemic and Diabetic Rats. JOURNAL OF ENDODONTICS, v. 45, n. 8, p. 1009-1015, AUG 2019. (13/23358-8)

ANGELO CINTRA, LUCIANO TAVARES; SAMUEL, RENATA OLIVEIRA; AZUMA, MARIANE MAFFEI; SILVA DE QUEIROZ, ANA OLIVIA; ERVOLINO, EDILSON; SUMIDA, DORIS HISSAKO; FELIX DE LIMA, VALERIA MARCAL; GOMES-FILHO, JOAO EDUARDO. Multiple Apical Periodontitis Influences Serum Levels of Cytokines and Nitric Oxide. JOURNAL OF ENDODONTICS, v. 42, n. 5, p. 747-751, MAY 2016. (13/23358-8)

SAMUEL, RENATA OLIVEIRA; GOMES-FILHO, JOAO EDUARDO; AZUMA, MARIANE MAFFEI; SUMIDA, DORIS HISSAKO; PENHA DE OLIVEIRA, SANDRA HELENA; CHIBA, FERNANDO YAMAMOTO; MOGAMI BOMFIM, SUELY REGINA; CIARLINI, PAULO CESAR; NARCISO, LUIS GUSTAVO; ANGELO CINTRA, LUCIANO TAVARES. Endodontic infections increase leukocyte and lymphocyte levels in the blood. CLINICAL ORAL INVESTIGATIONS, v. 22, n. 3, p. 1395-1401, APR 2018. (13/23358-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SAMUEL, Renata Oliveira. Local and systemic inflammatory profile of Wistar rats with multiple endodontics infections. 2016. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araçatuba Araçatuba.