Quantification of bacterial endotoxin in different types of orthodontic adhesive systems

• Grant number: 13/26611-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2014
• End date: December 31, 2014
• Field of knowledge: Health Sciences - Dentistry - Pediatric Dentistry
• Principal Investigator: Paulo Nelson Filho
• Grantee: Thais Rodrigues Guerra
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The aim of this study is to quantify in vitro the bacterial endotoxin (LPS) adhered to different orthodontic adhesive systems, using the Limulus Amebocyte Lysate test. Fifty specimens 3 mm diameter and 2 mm high will be manufactured employing frequently used adhesive systems' components for clinical fixation of orthodontic brackets. The Transbond XT and Reliance adhesive systems will be used, with 10 specimens for each component (resin or adhesive). Acrylic resin (Artigos Odontológicos Clássico Ltda.) samples will be used for control, five of them contaminated by LPS (positive control) and five uncontaminated (negative control). After sterilization in a dry depyrogenization sterilizer, the specimens will be contaminated by immersion in an endotoxin solution in Eppendorf-type non-pyrogenic plastic tubes. Only the Group IV specimens will be contamination-free (negative control). After the LPS contamination, the samples will be individually immersed in glass tubes containing pyrogen-free water under agitation to release LPS from the materials. Quantification of bacterial endotoxin (in UE/mL) will be performed by the Limulus Amebocyte Lysate test following the manufacturer's instructions. The obtained data will be adequately subjected to statistical analysis according to their distribution, with a 5% significance level.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ROMUALDO, PRISCILLA COUTINHO; GUERRA, THAIS RODRIGUES; ROMANO, FABIO LOURENCO; BEZERRA DA SILVA, RAQUEL ASSED; BRANDAO, IZAIRA TINCANI; SILVA, CELIO LOPES; BEZERRA DA SILVA, LEA ASSED; NELSON-FILHO, PAULO. Bacterial endotoxin adhesion to different types of orthodontic adhesives. Journal of Applied Oral Science, v. 25, n. 4, p. 436-441, JUL-AUG 2017. (13/26611-6)