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Galleria mellonella as non-vertebrate host infection model for the study of Porphyromonas gingivalis: analysis of virulence and sensitivity to photodynamic therapy

Grant number: 13/26820-4
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): April 01, 2014
Effective date (End): November 30, 2015
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Juliana Campos Junqueira
Grantee:Janaina Araújo de Alvarenga
Home Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil


The objective of this study will be to develop an in vivo model in Galleria mellonella for the study of Porphyromonas gingivalis, a strict anaerobic bacterium important for research in dentistry. A model for the study of P. gingivalis virulence and antimicrobial therapies tests will be developed, such as photodynamic therapy. To study the virulence different P. gingivalis concentrations will be inoculated into G. mellonella and the development of experimental infection will be evaluated by survival curve and hemocytes count, which are the immune cells present in the hemolymph of these animals. For the evaluation of G. mellonella as photodynamic therapy study model, toxicity tests will be performed with different concentrations of the methylene blue photosensitizer, as well as tests to determine the optimal parameters light irradiation (AsGaAl laser 660 nm). The following groups of G. mellonella larvae infected with P. gingivalis will be evaluated to four experimental conditions: inoculation of the photosensitizer and application of Laser (P+ L+), Physiologic solution inoculation and application of Laser (P-L +), inoculation the Photosensitizer without Laser (P+ L-) and inoculation of physiologic solution without Laser (P- L). The effects of PDT on infection by P. gingivalis will be evaluated by survival curve analysis of the larvae. The data obtained in G. mellonella survival curve will be analyzed by Log-rank method and hemocytes count by ANOVA test, considering a significance level of 5%.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ROSSONI, RODNEI DENNIS; DE BARROS, PATRICIA PIMENTEL; DE ALVARENGA, JANAINA ARAUJO; RIBEIRO, FELIPE DE CAMARGO; VELLOSO, MARISOL DOS SANTOS; FUCHS, BETH BURGWYN; MYLONAKIS, ELEFTHERIOS; CARDOSO JORGE, ANTONIO OLAVO; JUNQUEIRA, JULIANA CAMPOS. Antifungal activity of clinical Lactobacillus strains against Candida albicans biofilms: identification of potential probiotic candidates to prevent oral candidiasis. BIOFOULING, v. 34, n. 2, p. 212-225, 2018. Web of Science Citations: 17.
DOS SANTOS, JESSICA DIANE; DE ALVARENGA, JANAINA ARAUJO; ROSSONI, RODNEI DENNIS; GARCIA, MAIRA TERRA; MORAES, RENATA MENDONCA; ANBINDER, ANA LIA; CARDOSO JORGE, ANTONIO OLAVO; JUNQUEIRA, JULIANA CAMPOS. Immunomodulatory effect of photodynamic therapy in Galleria mellonella infected with Porphyromonas gingivalis. Microbial Pathogenesis, v. 110, p. 507-511, SEP 2017. Web of Science Citations: 8.

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