Transcription factors vitamin D receptor (VDR) and retinoid X receptor (RXR) genomic location analysis through chromatin immunoprecipitation

• Grant number: 14/03561-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2014
• End date: March 31, 2016
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Bruno Ferraz de Souza
• Grantee: Pedro Luís Flora da Costa
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 11/12696-4 - Functional genomic analysis of the transcriptional regulation by nuclear receptors in hereditary Vitamin D resistant rickets, AP.JP

Abstract

The genomic location of calcitriol-activated vitamin D receptor (VDR) and retinoid X receptor (RXR) will be investigated in control and p.Arg30* VDR-mutant fibroblasts, allowing the definition of calcitriol-VDR cistrome in this system and the identification of compensatory molecular mechanisms in the absence of a functional VDR.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

COSTA, PEDRO L. F.; FRANCA, MONICA M.; KATAYAMA, MARIA L.; CARNEIRO, EDUARDO T.; MARTIN, REGINA M.; FOLGUEIRA, MARIA A. K.; LATRONICO, ANA C.; FERRAZ-DE-SOUZA, BRUNO. Transcriptomic Response to 1,25-Dihydroxyvitamin D in Human Fibroblasts with or without a Functional Vitamin D Receptor (VDR): Novel Target Genes and Insights into VDR Basal Transcriptional Activity. CELLS, v. 8, n. 4, APR 2019. (11/12696-4, 14/03561-6)

COSTA, PEDRO L. F.; FRANCA, MONICA M.; FERRAZ-DE-SOUZA, BRUNO. Nonspecific binding of a frequently used vitamin D receptor (VDR) antibody: important implications for vitamin D research in human health. ENDOCRINE, v. 54, n. 2, p. 556-559, NOV 2016. (11/12696-4, 14/03561-6)