IDENTIFICATION OF MARKERS OF CELL CYCLE COMPETENCE INVOLVED IN THE CANINE OOCYTE PROTEOMIC ANALYSIS

• Grant number: 13/21667-3
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: May 01, 2014
• End date: August 31, 2017
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Maria Denise Lopes
• Grantee: Leda Maria Costa Pereira
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated scholarship(s): 15/17412-5 - Influence of EGF on the viability and activation of primordial follicles in ovarian tissue from prepubertal versus adults bitches, BE.EP.DR

Abstract

In mammals, oocytes are removed from the follicular environment, no longer have the signal that maintains meiotic block and restart meiosis. In bitches, although the oocyte meiosis can resume spontaneously in vitro maturation rates are very low and the percentage of oocytes that remain VG is high. Several studies have attempted to understand the cell cycle regulation in mammalian oocytes, especially in regulating a number of protein kinases and phosphatases which control meiosis in order to elucidate what remains blocked meiosis. Thus, this project aims to consolidate and extend the experimental study and applied, with emphasis on cell cycle control of canine oocytes and its association with oocyte competence in this species through molecular techniques refined to identify the expression of their target biomarkers. The ovaries will be collected from bitches considered otherwise healthy on clinical examination and submitted to ovariohysterectomy (OSH) elective. The oocytes will be selected for in vitro maturation (IVM) and assessed at 0h, 24h, 48h and 72h. The technique of proteomics will be used to identify and quantify the expression of proteins involved in cell cycle control and technique of immunofluorescence to assess the location of these proteins during the different stages of IVM.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

COSTA PEREIRA, LEDA MARIA; THONGKITTIDILOK, CHOMMANART; LOPES, MARIA DENISE; SONGSASEN, NUCHARIN. Effect of anti-apoptotic drug Z-VAD-FMK on in vitro viability of dog follicles. Theriogenology, v. 122, p. 124-129, DEC 2018. (14/19776-1, 13/21667-3, 15/17412-5)

PEREIRA, LEDA MARIA C.; BERSANO, PAULO RICARDO O.; ROCHA, DANILO D.; LOPES, MARIA DENISE. Effect of EGF on expression and localization of maturation-promoting factor, mitogen-activated protein kinase, p34(cdc2) and cyclin B during different culture periods on in vitro maturation of canine oocytes. REPRODUCTION IN DOMESTIC ANIMALS, v. 54, n. 2, p. 325-341, FEB 2019. (14/19776-1, 13/21667-3)

LEDA MARIA COSTA PEREIRA; PAULO RICARDO OLIVEIRA BERSANO; MARIA DENISE LOPES. INFLUÊNCIA DO HEPES NA MATURAÇÃO IN VITRO DE OÓCITOS CANINOS OBTIDOS DE CADELAS EM DIESTRO E ANESTRO. Ciênc. anim. bras., v. 20, 2019-12-13. (13/21667-3, 14/19776-1)