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Effect of low level laser on C2C12 muscle cells subjected to injury by BthTX-I and II myotoxin islalated from Bothrops jararacussu snake venom

Grant number: 13/23757-0
Support type:Scholarships in Brazil - Master
Effective date (Start): March 01, 2014
Effective date (End): February 28, 2015
Field of knowledge:Health Sciences - Pharmacy
Principal researcher:Stella Regina Zamuner
Grantee:Adriano Silvio dos Santos
Home Institution: Universidade Nove de Julho (UNINOVE). Campus Vergueiro. São Paulo , SP, Brazil


The venom of snakes of the Bothrops species, induces a local inflammatory reaction, characterized by pain, edema, leukocyte migration and can be accompanied by tissue necrosis. The use of antivenom performs the function of neutralizing the greatest possible amount of circulating venom, thus minimizing its systemic effects, but its action does not extend to local manifestations, and thus require the use of another therapeutic option to control this reaction. The low level laser therapy (LLLT) is an alternative treatment in cases of muscle injury due to its biological effects, such as analgesics, anitinflamatory and healing. In a previous study of our lab it was found that LBP can enhance the viability of C2C12 muscle cells after the addition of the venom of B. jararacussu in the medium and that this effect of LBP is related to protection of the cell membrane. This study will examine the effect of LBP, using the energy density 4J/cm2, wavelength » 660nm and 808 nm in the cell monolayer integrity, viability, differentiation of muscle cells and expression of myogenin in C2C12 cells, exposed to injury by myotoxins BthTX-I and II isolated from the venom of Bothrops jararacussu.Therefore, this study will better understand the pathophysiological effects of Bothrops envenoming, as well as participation of myotoxins in the local effect caused by Bothrops venom and contribute to a better understanding of the effects of low level laser and eventually promote therapeutics more efficient and/or complementary.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DOS SANTOS, ADRIANO SILVIO; GUIMARAES-SOUSA, LUDMILA; COSTA, MARICILIA SILVA; ZAMUNER, LUIS FERNANDO; SOUSA, NORMA CRISTINA; HYSLOP, STEPHEN; SOARES, ANDREIMAR M.; CHAVANTES, MARIA CRISTINA; COGO, JOSE CARLOS; ZAMUNER, STELLA REGINA. Photobiomodulation of local alterations induced by BthTX-I, a phospholipase A(2) myotoxin from Bothrops jararacussu snake venom: In vivo and in vitro evaluation. International Journal of Biological Macromolecules, v. 107, n. B, p. 2020-2025, FEB 2018. Web of Science Citations: 3.

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