Evaluation of genotoxicity of solamargine and solasonine glycoalkaloids and their influence on chromossomal damage induced by different mutagens

• Grant number: 13/22769-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): May 01, 2014
• Effective date (End): December 31, 2014
• Field of knowledge: Health Sciences - Pharmacy - Toxicological Analysis
• Principal Investigator: Denise Crispim Tavares Barbosa
• Grantee: Heloiza Diniz Nicolella
• Host Institution: Pró-Reitoria Adjunta de Pesquisa e Pós-Graduação. Universidade de Franca (UNIFRAN). Franca , SP, Brazil

Abstract

The ecosystem of the South America reveals a vast ecological diversity, so that the advance phytotherapic and the search for isolated molecules with biological activity in this region are of great potential, attracting pharmaceutical industries. Among the numerous species found in this natural variety, are species of the genus Solanum, which feature various biological activities, such as hypotensive, hepatoprotective, anti-inflammatory, antiviral, anti-allergic and antiurolitíase. Solanum lycocarpum. St-Hil, found in the Southeast and Center-West Brazil, stands for anti-epileptic activities, antispasmodic, as well as the significance in the treatment of diabetes, obesity, hepatitis and hemorrhoids. Solamargine (SM) and solasonine (SS) are the two main glycoalkaloids presents in S. lycocarpum and can be found in more than 100 species, being known to possess remarkable antitumor activity. In the face of the biological properties of the species of the genus Solanum and their glycoalkaloids SM and SS, the present study aims to assess the genotoxicity potential of glycoalkaloids and its influence on the DNA damage induced by different mutagens in Chinese hamster lung fibroblasts (V79 cells) by micronucleus test. For the assessment of genotoxicity, the cultures will be treated with 1.78; 3.55 and 7.1 µg/mL of SM and 3.6; 7.2 and 14.4 µg/mL of SS. These concentrations of SM and SS are associated with two different mutagens, etoposide (VP16 1 µg/mL; topoisomerase II inhibitor) and camptotecin (CPT 43 µg/mL; topoisomerase I inhibitor). Control groups will also be included (negative -without treatment; positive - VP16 and CPT; and solvent - dimethylsulfoxide). For the analyzed parameter, the frequency of micronucleus will be analyzed in 3000 binucleated cells by treatment group. The cytotoxicity of the treatments will also be evaluated by the nuclear division index (IDN) where 1500 cells per treatment will be counted. The study will allow better understanding about the mechanisms of action of these glycoalkaloids well as the species S. lycocarpum.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NICOLELLA, HELOIZA DINIZ; DE OLIVEIRA, POLLYANNA FRANCIELLI; MUNARI, CARLA CAROLINA; DIAS COSTA, GIZELA FALEIROS; MOREIRA, MONIQUE RODRIGUES; SOLA VENEZIANI, RODRIGO CASSIO; TAVARES, DENISE CRISPIM. Differential effect of manool - A diterpene from Salvia officinalis, on genotoxicity induced by methyl methanesulfonate in V79 and HepG2 cells. Food and Chemical Toxicology, v. 72, p. 8-12, OCT 2014. (13/22769-4, 11/05732-4)