Efect of lower laser teraphy after rapid maxillary expansion in c-Fos expression

• Grant number: 13/16569-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: May 01, 2014
• End date: December 31, 2014
• Field of knowledge: Health Sciences - Dentistry - Orthodontics
• Principal Investigator: Maria Bernadete Sasso Stuani
• Grantee: Laura Borges
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Objective: The aim of the present in vivo study will be to quantitatively evaluate the pain control after rapid maxillary expansion (RME) in young rats, analyzing the activation of nociceptive related structures, using c-fos expression in caudalis, interpolaris, and oralis sub nuclei. MATERIALS and Methods: a total of 50 rats Wistar (Rattus norvegicus, albinus), male, weighing 220g, were assigned to 2 groups: Control Group (n=25) with no treatment; Experimental I (n=25) with RME. The animals will be euthanized at hours 6, 12, 24, 48 and 72 after RME; . The animals will be euthanized at the same periods described for Experimental I. After the experimental periods, they will be anesthetized, and perfused with 4% paraformaldehyde. In the sequence, brains will be removed from the skull and processed for FOS immunocytochemistry. Coronal sections through the spinal trigeminal caudalis (SpVc-bregma: -15.96 and -14.28 mm), spinal trigeminal interpolaris (SpVi-bregma: -13.32 mm), and spinal trigeminal oralis (SpVo-bregma: -11.28 mm) will be cut at 40µm thickness on a cryostat. Every third section will be collected in PBS and processed for Fos immunohistochemistry. The Image J software will be also used to quantify Fos-IR neurons in sections of the rat brains. The sections will be analyzed light microscopically (10× objective). The ANOVA and Tukey tests will be used and significance level was set at 5%.