Impact of androgens on the expression of inflammatory cytokines and markers of tissue repair systemically and on periodontal tissues of female rats under conditions of periodontal health, disease and repair

• Grant number: 14/02280-3
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2014
• End date: July 31, 2016
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Luis Carlos Spolidorio
• Grantee: Daniel Olivio Ribeiro
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

The etiopathogenesis of the immune-inflammatory responses associated with periodontal disease is a strictly regulated and complex process that involves many molecules related to different cell signaling pathways. In this context, the interaction of hormones and their receptors, including sex hormones, plays an important role. It is plausible that androgenic hormones' role in immune-inflammatory responses is based on a complex interaction network that culminates in host response modulation. Particularly, our group demonstrated that sub- and supraphysiologic androgen levels exacerbate the immune-inflammatory response and periodontal bone loss using a ligature-induced periodontitis model in male rats. Besides, we reported that physiologic levels of testosterone inhibit osteoclastogenesis both directly and indirectly, reducing osteoblast-derived RANKL expression in vitro. Despite the biological plausibility in the participation of androgenic hormones playing an active role in the etiopathogenesis of periodontal diseases, little is known about the importance of androgens on the different events that take place during the progression and reparative phases of periodontal disease in females. This project aims to extend our studies inhibiting androgen and estrogen receptors or administering testosterone with or without aromatase inhibitors in female rats. We will evaluate the inflammatory profile by analysis of expression of IL-1², IL-2, IL-4, IL-6, TNF-± e PgE2 and indicators of tissue repair through the expression of VEGF, PDGF, CXCL4, and CCL5, by ELISA, systemically and locally. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

STEFFENS, JOAO PAULO; LEAL SANTANA, LUIS CARLOS; PAIVA PITOMBO, JONLENO COUTINHO; RIBEIRO, DANIEL OLIVIO; COSTA ALBARICCI, MARIA CAROLINA; CUBAS WARNAVIN, STEPHANIE VON STEIN; KANTARCI, ALPDOGAN; SPOLIDORIO, LUIS CARLOS. The role of androgens on periodontal repair in female rats. Journal of Periodontology, v. 89, n. 4, p. 486-495, APR 2018. (13/07833-8, 14/02280-3, 13/23116-4, 13/12014-6)