Study and molecular characterization of Uropathogenics Escherichia coli strains and its potential as pathogenic strains for poultry (APEC)

• Grant number: 14/04860-7
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: June 01, 2014
• End date: September 30, 2017
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Wanderley Dias da Silveira
• Grantee: Janaína Luisa Leite Garbim
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

The Brazil is currently the third largest producer of poultry meat, the largest exporter, which demonstrates the importance of the poultry industry for domestic and foreign economies, creating jobs, high protein foods with low cost and currency. Infections caused by pathogens such as viruses, fungi and bacteria such as Escherichia coli, are agents that, in addition to affecting the poultry industry, causing reduced productivity and thus increasing cost, they cause of morbidity and mortality birds. Studies have shown that some extraintestinal pathogenic strains (ExPEC) E. coli to humans, such as Uropathogenic (UPEC) and causing meningitis in newborns (NEMEC) and birds (Avian Pathogenic E. coli-APEC) share some virulence-related genes, which would make the latter present zoonotic potential may act as human ExPEC or as a reservoir of virulence genes for human ExPEC. In this work, we intend to perform molecular characterization of three strains of UPEC causing urinary tract infections (UTIs) in humans, analyzing the virulence factors present in them, as well as gene expression, through experiments in vitro and in vivo poultry and thus determine its potential pathogenic for poultry.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VERMA, RENU; GALVAO ROJAS, THAIS CABRERA; MALUTA, RENATO PARIZ; LEITE, JANAINA LUISA; NAKAZATO, GERSON; DE SILVEIRA, WANDERLEY DIAS. Role of hypothetical protein YicS in the pathogenicity of Avian Pathogenic Escherichia coli in vivo and in vitro. MICROBIOLOGICAL RESEARCH, v. 214, p. 28-36, 2018. (12/23289-3, 12/09655-7, 12/05073-3, 15/03860-6, 13/09167-5, 14/04860-7)

VERMA, RENU; GALVAO ROJAS, THAS CABRERA; MALUTA, RENATO PARIZ; LEITE, JANANA LUISA; MENDES DA SILVA, LIVIA PILATTI; NAKAZATO, GERSON; DA SILVEIRA, WANDERLEY DIAS. Fimbria-Encoding Gene yadC Has a Pleiotropic Effect on Several Biological Characteristics and Plays a Role in Avian Pathogenic Escherichia coli Pathogenicity. Infection and Immunity, v. 84, n. 1, p. 187-193, JAN 2016. (12/23289-3, 12/04391-1, 12/05073-3, 13/09167-5, 14/04860-7)