Evaluation of cell behavior induced by endodontic sealers

• Grant number: 14/08671-4
• Support Opportunities: Scholarships abroad - Research Internship - Scientific Initiation
• Start date: July 11, 2014
• End date: August 10, 2014
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Léa Assed Bezerra da Silva
• Grantee: Leopoldo Ulian Azevedo
• Supervisor: Ricardo A. Battaglino
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Institution abroad: Harvard University, Boston, United States
• Associated to the scholarship: 13/21180-7 - Evaluation of cell behavior and response of subcutaneous connective tissue of isogenic mice induced by endodontic sealers, BP.IC

Abstract

The aim of this study is to evaluate cell behavior induced by sealers Real Seal XT1 and Sealapex Xpress. The cell viability and proliferation will be assessed after exposure of cells to cement Sealapex Spress (group I) and Real Seal XT1 (group II). As a control, cells will be exposed to bacterial endotoxin (LPS) (Group III) and to the DMEM-c culture (Group IV). Cell viability of the suspension of RAW 264.7 macrophage lineage will be evaluated in different groups by MTT colorimetric assay (Sigma). Cell viability in groups I and II will be expressed in percentage and compared to groups III and IV (controls). Cell proliferation will be determined by immunostaining the Ki-67 nuclear antigen expressed only by cells during the cell cycle and not by cells in G0. The result will be expressed as the total number of cells per well. The proportion of cells that exhibit changes in the cytoplasmic membrane will be determined by flow cytometry from 1 to 10 days. The readings will be held in flow cytometry and apoptosis will be expressed in percentage compared to groups III and IV. Numerical data will be analyzed using the appropriate test according to the nature of the data. The significance level will be 0,05. (AU)