Evaluation of sedation and analgesia provided by morphine in tegu (Salvator merianae)

• Grant number: 14/03247-0
• Support Opportunities: Scholarships in Brazil - Master
• Start date: June 01, 2014
• End date: September 30, 2015
• Field of knowledge: Biological Sciences - Zoology - Animal Behavior
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Adriano Bonfim Carregaro
• Grantee: William Petroni Leal
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil

Abstract

The study aims to quantify sedation and analgesia of morphine in tegu (Salvator merianae). Ten male and female young tegus weighing between 100 and 500gr will be submitted to three treatments: G10 - 10mg/kg of morphine by intramuscular route (IM); G20 - 20mg/kg of morphine IM, and G0 - which will receive 0.5 mL of saline solution IM. Sedation will be evaluated by locomotor activity, using an open-field test. Animals will be placed on the open-field center and it will be scored when animal overtakes the division areas, in 5 minutes. Locomotor activity will be measured at 0 min (baseline), 30 min, 1, 2, 3, 4, 6, 12 and 24 hours after treatments. Analgesia of morphine will be evaluated by pedal with draw reflex, using a thermal stimulus located under the plantar surface. Animals will be placed into a plastic box, 10 minutes before the stimulus, for adaptation. Afterwards, animals will receive a 45-47°C thermal stimulus at 0 min (baseline), 30 min, 1, 2, 3, 4, 6, 12 and 24 hours after treatments. Tegus behavior will be recorded by video camera and they will be analyzed by two observers, whom unknown the treatments. It is expected to establish the best dose of morphine to promote adequate analgesia and some degree of sedation in tegus. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LEAL, WILLIAM P.; CARREGARO, ADRIANO B.; BRESSAN, THAIS F.; BISETTO, SHAYNE P.; MELO, CRISTIANO F.; SLADKY, KURT K.. Antinociceptive efficacy of intramuscular administration of morphine sulfate and butorphanol tartrate in tegus (Salvator merianae). AMERICAN JOURNAL OF VETERINARY RESEARCH, v. 78, n. 9, p. 1019-1024, SEP 2017. (14/10452-9, 14/03247-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

LEAL, William Petroni. Sedative and analgesic evaluations of morphine in tegus (Salvator merianae). 2015. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Zootecnica e Engenharia de Alimentos (FZE/BT) Pirassununga.