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Characterization of opioid receptors in the central nervous system and evaluation of analgesic and sedative effects of opioids in tegus (Salvator merianae) and green iguanas (Iguana iguana)

Grant number: 14/10452-9
Support Opportunities:Regular Research Grants
Duration: November 01, 2014 - April 30, 2017
Field of knowledge:Biological Sciences - Zoology - Animal Behavior
Principal Investigator:Adriano Bonfim Carregaro
Grantee:Adriano Bonfim Carregaro
Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
Associated researchers:Augusto Shinya Abe ; Heidge Fukumasu


The aims of this study are to characterize and to localize opioid receptors (OR) in the Central Nervous System (CNS) of tegus (Salvator merinae) and green iguanas (Iguana iguana), to quantify sedative and analgesic effects of morphine and butorphanol at different doses in those animals, and to evaluate the influence of the seasonality on those tests. Characterization of the OR in the CNS will be perform by cloning and amplification of the OR cDNA, using 3` e 5` RACE PCR, to create a nucleotide sequence referent to MOR and KOR. OR cDNA will be used to detect and to localize those receptors in the CNS. This part will be performed by immunohistochemistry technique. To the second and third phase, 10 young animals of each specie, male or female, will be submitted to five treatments: morphine 10mg/kg (GM10), morphine 20mg/kg (GM20), butorphanol 10mg/kg (GB10), butorphanol 20mg/kg (GB20) and 0.5 mL of NaCl 0.9% (GC), by intramuscular route. Spontaneous locomotor activity will be performed before treatments (basal), 30 minutes, 1, 2, 3, 4, 6, 12 e 24 hours after treatments, by an open field technique. Analgesia will be performed at the same times described before, on another time, by noxious thermal stimulation (45 - 47°C). It will be expected to get the characterization and the localization of the MOR and KOR in the CNS of those animals, as well as to determine sedative and analgesic effects of morphine and butorphanol. Besides, it will be expected to determine the best morphine and butorphanol doses for those species. (AU)

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BISETTO, SHAYNE P.; MELO, CRISTIANO F.; CARREGARO, ADRIANO B.. Evaluation of sedative and antinociceptive effects of dexmedetomidine, midazolam and dexmedetomidine-midazolam in tegus (Salvator merianae). VETERINARY ANAESTHESIA AND ANALGESIA, v. 45, n. 3, p. 320-328, . (14/10452-9)
BRESSAN, THAIS F.; SOBREIRA, THAYANEE; CARREGARO, ADRIANO B.. Use of Rodent Sedation Tests to Evaluate Midazolam and Flumazenil in Green Iguanas (Iguana iguana). Journal of the American Association for Laboratory Animal Science, v. 58, n. 6, p. 810-816, . (14/10452-9)
LEAL, WILLIAM P.; CARREGARO, ADRIANO B.; BRESSAN, THAIS F.; BISETTO, SHAYNE P.; MELO, CRISTIANO F.; SLADKY, KURT K.. Antinociceptive efficacy of intramuscular administration of morphine sulfate and butorphanol tartrate in tegus (Salvator merianae). AMERICAN JOURNAL OF VETERINARY RESEARCH, v. 78, n. 9, p. 1019-1024, . (14/10452-9, 14/03247-0)
ANDRÉ NICOLAI ELIAS DA SILVA; THAIS FERES BRESSAN; ADRIANO BONFIM CARREGARO. Mensuração da pressão intracelomática e da pressã de perfusão celomática em teiús saudáveis (Salvator merianae). Ciência Rural, v. 48, n. 2, . (14/10452-9)

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