Evaluation of myocardial contractility and myocyte function of neonate rabbits with congenital diaphragmatic hernia created surgically

• Grant number: 14/02519-6
• Support Opportunities: Scholarships abroad - Research
• Start date: September 15, 2014
• End date: September 14, 2015
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Lourenço Sbragia Neto
• Grantee: Lourenço Sbragia Neto
• Host Investigator: Jose Luis Peiró Ibáñez
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Institution abroad: Cincinnati Children's Hospital Medical Center, United States

Abstract

Congenital diaphragmatic hernia is a defect in the development of the diaphragm that occurs in 1:2500 newborns with high fetal and neonatal mortality secondary to pulmonary hypoplasia and hypertension. This defect can be experimentally created in rabbit fetuses at 25 days of gestation with 50% of fetal survival. Histologically, the lungs in CDH show disturbed alveolarization and vascularization leading to respiratory failure and difficulties in ventilating the newborn. Besides, the pulmonary hypertension increases the right ventricular, which has implications on follow up progress and survival. Our objective is to investigate the cardiac function and myocardial contractility immediately after delivery in the surgical model of CDH. We will study the in vitro contractility of the myocardium from the left and right ventricles and compare it to control fetuses. Following, we will study the effect of vasoactive drugs on the myocardium and its possible implications to pulmonary hypertension and myocardial function in CDH. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PEIRO, JOSE L.; SBRAGIA, LOURENCO; SCORLETTI, FEDERICO; LIM, FOONG Y.; SHAABAN, AIMEN. Management of fetal teratomas. PEDIATRIC SURGERY INTERNATIONAL, v. 32, n. 7, p. 635-647, JUL 2016. (14/02519-6)

LANHELLAS GONCALVES, FRANCES LILIAN; FIGUEIRA, REBECA LOPES; GALLINDO, RODRIGO MELO; BERTONCINI SIMOES, ANA LEDA; COLEMAN, ALAN; PEIRO, JOSE LUIS; SBRAGIA, LOURENCO. Tracheal occlusion and ventilation changes the nitric oxide pathway in congenital diaphragmatic hernia model. JOURNAL OF SURGICAL RESEARCH, v. 203, n. 2, p. 466-475, JUN 15 2016. (11/12587-0, 11/00794-1, 14/02519-6)

VARISCO, BRIAN M.; SBRAGIA, LOURENCO; CHEN, JING; SCORLETTI, FEDERICO; JOSHI, RASHIKA; WONG, HECTOR R.; LOPES-FIGUEIRA, REBECA; ORIA, MARC; PEIRO, JOSE. Excessive Reversal of Epidermal Growth Factor Receptor and Ephrin Signaling Following Tracheal Occlusion in Rabbit Model of Congenital Diaphragmatic Hernia. Molecular Medicine, v. 22, p. 398-411, 2016. (14/02519-6)

SCHMIDT, AUGUSTO FREDERICO; LANHELLAS GONCALVES, FRANCES LILIAN; FIGUEIRA, REBECA LOPES; SCORLETTI, FEDERICO; PEIRO, JOSE LUIS; SBRAGIA, LOURENCO. Combined antenatal therapy with retinoic acid and tracheal occlusion in a rat model of congenital diaphragmatic hernia. PEDIATRIC SURGERY INTERNATIONAL, v. 32, n. 6, p. 591-598, JUN 2016. (11/12587-0, 11/00794-1, 14/02519-6)

SBRAGIA, LOURENCO; ORIA, MARC; SCORLETTI, FEDERICO; ROMERO LOPEZ, MARIA DEL MAR; SCHMIDT, AUGUSTO F.; LEVY, BRITTANY; PEIRO, JOSE L.. A novel surgical toxicological-free model of diaphragmatic hernia in fetal rats. Pediatric Research, AUG 2021. (14/02519-6)