Identification of microRNAs differentially expressed in serous carcinomas of high-grade patients with different clinical outcomes

• Grant number: 14/03021-1
• Support type: Scholarships in Brazil - Doctorate
• Effective date (Start): June 01, 2014
• Effective date (End): January 16, 2018
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal researcher: Rafael Malagoli Rocha
• Grantee: Nayra Soares Do Amaral
• Home Institution: A C Camargo Cancer Center. Fundação Antonio Prudente (FAP). São Paulo , SP, Brazil
• Associated scholarship(s): 16/09349-4 - Validation and functional studies of differentially expressed miRNAs in high grade serous carcinoma of patients with different clinical developments, BE.EP.DR

Abstract

Introduction: The epithelial ovarian cancer is the fifth leading cause of cancer death among women. The molecular processes that influence the prognosis of patients with ovarian cancer are not understood, therefore it is necessary to identify molecules that enable the understanding of the molecular mechanisms involved in this type of tumor, such as miRNAs. Although many studies suggest the importance of miRNAs in the development of ovarian cancer, the differential expression of these molecules are poorly studied in patients with good or poor prognosis. Objective: To characterize the expression profile of miRNAs in samples of serous ovarian carcinomas in patients with good and poor prognosis. Methods: Forty samples of serous ovarian carcinoma will be selected, including twenty samples with good prognosis and twenty with poor prognosis. Ten normal samples will be microdissected and will be used as global non-tumor control pool. The identification of differentially expressed miRNAs in the samples will be performed by qRT-PCR using the TaqMan system cards human microRNA array 2.0 A + B. Also will be performed to validate the selected fabric fresh miRNAs, in silico search for potential gene targets of these miRNAs by algorithms such as miRWalk, Miranda, PicTar, miRBase and TargetScan and testing biological activity of miRNAs elected in culture ovarian cell, as well as evaluating the gene expression of targets of these miRNAs by qRT-PCR and protein by immunocytochemistry. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DO AMARAL, NAYRA SOARES; CRUZ E MELO, NATALIA; MAIA, BEATRIZ DE MELO; ROCHA, RAFAEL MALAGOLI. Noncoding RNA Profiles in Tobacco- and Alcohol-Associated Diseases. GENES, v. 8, n. 1 JAN 2017. Web of Science Citations: 4.