Assessment of the effects of exposure to Pb through respiration and feeding in blue crabs Callinectes danae

• Grant number: 14/01576-6
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: August 01, 2014
• End date: November 30, 2017
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Inorganic Chemistry
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Denis Moledo de Souza Abessa
• Grantee: Isabella Cristina da Costa Leal Bordon
• Host Institution: Instituto de Biociências (IB-CLP). Universidade Estadual Paulista (UNESP). Campus Experimental do Litoral Paulista. São Vicente , SP, Brazil

Abstract

The main hydrographic basin that contributes to the Cananéia-Iguape-Peruíbe estuarine complex - CIP (represented by the Ribeira de Iguape River basin - RIR) was place of intense activity of mining and metallurgy of lead (Pb) in the past. Although mining activities have been closed, they still influence the RIR and CIP, as slags and tailing piles are deposited on the river banks and are a source of metals to the system. Environmental assessments have focused on determining the concentrations of Pb in water, sediments and native biota, but there is little information on the effects of Pb to native organisms. Biomonitors can be used to evaluate environmental contamination, but require the understanding of the contaminants uptake mechanisms by the organisms, including the main pathways and the internal regulatory mechanisms of contaminants. Blue crabs of the genus Callinectes, commonly found in estuarine regions of São Paulo, have been used as environmental biomonitors. This study aims to evaluate the effects of Pb exposure through feeding and respiration in the blue crab Callinectes danae from the CIP region. Blue crabs will be collected in the South portion of CIP, and will be used in experiments of exposure to Pb by aqueous and feeding routes, for 7 and 15 days. Two Pb concentrations will be used (0.5 and 2.0 mg/Kg), with 4 treatments: control, aqueous exposure, feeding exposure, combined exposure. At the end of bioassays, tissues of 5 individuals per group will be removed and analyzed for contamination and biomarkers. Statistical and multivariate tools will be used to analyze the data. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BORDON, ISABELLA C.; JOVIANO, WILLIAN REINA; DE MEDEIROS, ALINE MARIA ZIGIOTTO; DE CAMPOS, BRUNO GALVAO; ARAUJO, GIULIANA SERAPHIM DE; GUSSO-CHOUERI, PALOMA KACHEL; PRETO, MAYRA DE FREITAS; FAVARO, DEBORAH INES TEIXEIRA; ABESSA, DENIS MOLEDO DE SOUZA. Heavy Metals in Tissues of Blue Crabs Callinectes danae from a Subtropical Protected Estuary Influenced by Mining Residues. Bulletin of Environmental Contamination and Toxicology, v. 104, n. 4, MAR 2020. (14/01576-6)

BORDON, ISABELLA C.; DE CAMPOS, BRUNO GALVAO; GUSSO-CHOUERI, PALOMA KACHEL; MIYAI, CAIO AKIRA; DE ARAUJO, GIULIANA SERAPHIM; EMERENCIANO, ANDREWS KRUPINSKI; MACHADO CUNHA DA SILVA, JOSE ROBERTO; BARBOZA COTRIM, MARYCEL ELENA; TEIXEIRA FAVARO, DEBORAH INES; DE SOUZA ABESSA, DENIS MOLEDO. Improvements in metal exposure assays: artificial food to assess bioaccumulation in the blue crab Callinectes danae Smith, 1869 (Crustacea, Decapoda, Portunidae). International Journal of Environmental Research, v. 13, n. 2, p. 431-434, APR 2019. (14/01576-6)

BORDON, ISABELLA C.; EMERENCIANO, ANDREWS KRUPINSKI; COSTA MELO, JULIA REALI; MACHADO CUNHA DA SILVA, JOSE ROBERTO; TEIXEIRA FAVARO, DEBORAH INES; GUSSO-CHOUERI, PALOMA KACHEL; DE CAMPOS, BRUNO GALVAO; DE SOUZA ABESSA, DENIS MOLEDO. Implications on the Pb bioaccumulation and metallothionein levels due to dietary and waterborne exposures: The Callinectes danae case. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, v. 162, p. 415-422, OCT 30 2018. (14/01576-6)