Osteoclast differentiation, nodule formation, general bone and periapical lesion phenotype in BPH/2 strain mice

• Grant number: 14/10541-1
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: September 15, 2014
• End date: July 08, 2015
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: João Eduardo Gomes Filho
• Grantee: Christine Men Martins
• Supervisor: Hajime Sasaki
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil
• Institution abroad: Forsyth Institute, United States
• Associated to the scholarship: 13/09446-1 - Influence of hypertension in tissue response and mineralization capacity of cement repairers MTA Angelus® white and grey and IRM®. Histology and immunohistochemistry analysis in rats., BP.DR

Abstract

Hypertension may cause an increased calcium mobilization from bone and its excretion by the kidneys. Patients with systemic disease may present reduction on bacterial infection resistance and tissue repair after endodontic. Thus, the aim of this study will be to analyze the osteoclast differentiation from bone marrow cells, the nodule formation using calvaria-derived osteoblasts and the general bone and periapical lesion phenotype from BPH/2 strain mice, comparing with its normal control. For this, an in vitro study will be performed. Bone marrow cells will be isolated from the tibiae and femora of BPH/2 strain mice and its control and specific cultured. Osteoclast formation will be evaluated by measuring the tartrateresistant acid phosphatase (TRAP) activity as an early differentiation marker and counting the number of TRAP-positive multinucleated cells with more than three nuclei after TRAP staining. Furthermore, osteoblasts calvaria derived from BPH/2 strain mice and its control will be isolated and culturing and the mineralization will be assessed by staining with Alizarin red. An in vivo study also will be performed. The dental pulps of the first mandibular right molar will be exposed and a periapical lesion will be stimulated. It will not do the same on the first mandibular left molar. On days 1, 10, and 21 after pulp exposure, all groups of mice will be killed and mandibles will be isolated and dissected free of soft tissues. Both right and left hemimandibles will be fixed in fresh 4% paraformaldehyde in PBS and will be used for histological and immunohistochemical analysis. The results will be statistically analyzed. (AU)