Assemblage and comparative analysis of phytopathogenic fungi genomes of Moniliophthora (Agaricales, Basidiomycota) obtained by large-scale sequencing using high-performance computational methods

• Grant number: 14/09638-0
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Effective date (Start): August 01, 2014
• Effective date (End): May 31, 2016
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Gonçalo Amarante Guimarães Pereira
• Grantee: Juliana José
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 13/08293-7 - CCES - Center for Computational Engineering and Sciences, AP.CEPID

Abstract

Moniliophthora perniciosa and M. roreri are among the few Agaricales species that evolved a phytopatogenecity life style. Both species are cacao (Theobroma cacao L.) pathogens, responsible for two serious and economically relevant diseases: witch's broom and pod rot. The presence of only few plant pathogens on related groups, the relative short divergence time, and the high variability found among M. perniciosa biotypes, turn this group into a potential model for either the study of genomic changes associated to the evolution of plant patogenicity and the future development to strategies against plant pathogens. In order to get a whole knowledge about genes related to the development of plant patogenicity, it is imperative to incorporate an evolutionary perspective on genomic analysis. These kind of analysis was applied on a previous project in the search for expansion and retraction signals on gene families of Moniliophthora when comparing to species of phylogenetically related groups. In the present project we propose a deeper analyses on genomic mechanisms for pathogenecity, adding to previous data the genome assembly of isolates from biotypes C and S of M. perniciosa obtained with high throughput sequencing and analysing comparatively the evolutionary rates of coding regions within and among species of this fungi group. Computing methods will be applied for a genomic scale scan for genes and regions of high evolutionary rates and also for a detailed analysis of genes highlighted both on the genomic scan and on the families with significant expansion or retraction detected on previous results.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NASCIMENTO, LEANDRO COSTA; YANAGUI, KARINA; JOSE, JULIANA; CAMARGO, EDUARDO L. O.; GRASSI, MARIA CAROLINA B.; CUNHA, CAMILA P.; BRESSIANI, JOSE PRIME ANTONIO; CARVALHO, GUILHERME M. A.; CARVALHO, CARLOS ROBERTO; PRADO, PAULA F.; et al. Unraveling the complex genome of Saccharum spontaneum using Polyploid Gene Assembler. DNA Research, v. 26, n. 3, p. 205-216, JUN 2019. (13/08293-7, 14/09638-0, 12/05890-1)