Protein diets on the activity of digestive enzymes in eggs and larvae of tilapia, Oreochromis niloticus

• Grant number: 14/15194-8
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree
• Start date: October 09, 2014
• End date: April 08, 2015
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
• Principal Investigator: Elizabeth Romagosa
• Grantee: Mayara de Moura Pereira
• Supervisor: Enric Gisbert Casas
• Host Institution: Centro de Aquicultura (CAUNESP). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Institution abroad: Investigación y Tecnología Agroalimentarias, Tarragona (IRTA), Spain
• Associated to the scholarship: 13/22570-3 - Digestive enzymes in embryogenesis of Nile Tilapia, Oreochromis niloticus, GIFT strain, BP.MS

Abstract

The digestive digestive enzymes reflect the characteristics of the fish and affect digestion and absorption of food. This study aimed to evaluate the activity of digestive enzymes (trypsin, pepsin, alanine phosphatase, amylase, lipase and leucine-alanine peptidase) in the embryonic and larval stages of breeding of Nile tilapia, GIFT strain, fed levels of protein diets. The experiment was conducted in Campus Center East APTA - UPD, Pirassununga, SP, Brazil. The experiment was composed of 16 hapas (1.5 X 3.0 X 1.0 m) with nine females and three males in each hapa (average weight 300g) will be stored. The experimental design was completely randomized with four treatments and four replications, with a control treatment, Tr1 (32% CP) and 3 others Tr2 (38% CP), Tr3 (44% CP) and Tr4 (50% CP). Males and females received the experimental diets 30 days prior to project implementation and during the experimental period (four months: January to April). Every two weeks, during the experiment, breeders were put together and two days after the union checked for the presence of eggs in the mouth of all females. When detected, they were conducted to the weight (g), to quantify the volume and placed in incubators (5L) for monitoring embryo development, where in each period (T0-cleavage, T1-blastula, gastrula-T2 and T3-hatching) and larval period were two periods (T4-T5 and 7 days - 10 days old) were stored 300mg/fêmea of eggs and larvae for analysis of digestive enzymes. Were collected in total 96 samples, 24 per treatment and four for each stage to quantify the enzymes. The analysis will be performed in the laboratory of IRTA, Sant Carles de La Ràpita, Spain, until analyzes are performed, the samples will be kept in liquid nitrogen. Results will be compared by treatment and subjected to analysis of variance and Tukey's test (5%) (SAS, version 9.0). (AU)