| Grant number: | 16/13883-6 |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| Start date: | October 01, 2016 |
| End date: | July 31, 2017 |
| Field of knowledge: | Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture |
| Principal Investigator: | Leonardo Tachibana |
| Grantee: | Amanda Iracy Cavalcante da Silva |
| Host Institution: | Instituto de Pesca. Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). São Paulo , SP, Brazil |
| Associated scholarship(s): | 16/24599-7 - Possible alterations in intestinal microbiota promoted by probiotic Aqua- photo® administration in Nile tilapia (Oreochromis niloticus), BE.EP.IC |
Abstract This project aim to evaluate the activity of digestive enzymes of Nile tilapia (Oreochromis niloticus) fed diets containing 0.08% of commercial probiotic AQUA-PHOTO which is composed by Lactobacillus plantarum (1.51x106 UFC/g) and Bacillus subtilis (1.34x107 UFC/g). The experimental design will be completely randomized with two treatments and 16 replicates (each fish will be considered as one replicate). The 48 sex reversed Nile tilapias (mean weight of 30 g) will be distributed in six aquaria with volume capacity of 60 L (three aquaria will receive control diet and three diet with probiotic). After seven days of adaptation with experimental diets and, two hours after feeding, eight specimens of each aquaria will be collect to intestine extraction (anterior, medial and posterior) to evaluate the enzymatic activities of amylase, lipase, alkaline protease and alkaline phosphatase. For these enzymes extraction, the intestine samples will be homogenized in phosphate buffer solution (PBS), centrifuged at velocity of 13.000 xg during three minutes at 4°C and supernatant will be used to determine the enzymatic activity. The amylase and alkaline protease analysis will follow the Bernfeld (1950) and Walter (1984) modified methodologies, respectively. For lipase and alkaline phosphatase will be determined by specific kits (BioclinTM). The results will be statistically evaluated by ANOVA (variance analysis) and means will be compared by Tukey Test (p<0.05). We expected beneficial effects of probiotic added on Nile tilapia feed in order to improve the digestive enzymes activities. (AU) | |
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