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Production of fungal phytases and proteases, feed application and digestible values for Nile tilapia

Grant number: 13/02998-9
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): June 01, 2013
Effective date (End): July 31, 2015
Field of knowledge:Agronomical Sciences - Animal Husbandry - Animal Nutrition and Feeding
Principal Investigator:Luciana Francisco Fleuri
Grantee:Paula Kern Novelli
Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil


The use of enzymes in animal feed is common practice nowadays and the use of exogenous microbial enzymes has shown good results for improving the efficiency of nutrients utilization. The ingredients used for fish feed formulation aim to provide nutrients to meet their requirements, and affect the availability in the animal intestinal tract. In this sense, the goal of this work is the production of proteases and phytases from microorganisms for diet application to improve nutritional availability and reduce antinutritional elements and evaluation of digestible values for Nile tilapia. The enzyme production will be prepared by Solid State Fermentation (SSF) using fungi belonging to the collection of microorganisms from Microorganisms Laboratory, Department of Chemistry and Biochemistry, UNESP - Botucatu and microorganisms offered by Osvaldo Cruz Foundation with soybean meal as substrate. It will be analyzed the enzymatic activities for phytase and protease in the laboratory to select the best fungus for enzyme production and concentrated enzyme extract will be added to diets with soybean meal or corn gluten as test ingredients for further analysis of digestibility using chromium III oxide as external marker. Tilapia juveniles will be distributed in six completely randomized treatments in a factorial with two protein sources (soybean meal or corn gluten) and inclusion of enzymes (protease, phytase and protease plus phytase). Analysis of variance test will be used and, when there is difference between averages, it will be applied the Tukey test with 5% significance for all the experiments conducted in this project.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
NOVELLI, PAULA K.; BARROS, MARGARIDA M.; PEZZATO, LUIZ EDIVALDO; DE ARAUJO, ERIC PORTILHO; BOTELHO, RENAN DE MATTOS; FLEURI, LUCIANA F.. Enzymes produced by agro-industrial co-products enhance digestible values for Nile tilapia (Oreochromis niloticus): A significant animal feeding alternative. Aquaculture, v. 481, p. 1-7, . (13/02998-9)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
NOVELLI, Paula Kern. Screening of fungi phytases produced by solid state fermentation with different agricultural by-products as substrate. 2015. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu Botucatu.

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