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Amylolytic and fibrolytic enzymes in feeding dairy cows

Grant number: 16/15711-8
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): June 01, 2017
Effective date (End): February 28, 2018
Field of knowledge:Agronomical Sciences - Animal Husbandry - Animal Nutrition and Feeding
Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Francisco Palma Rennó
Grantee:Elissandra Maiara de Castro Zilio
Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil


The use of additives in the feeding of ruminants has been practice to improve the efficiency and animal performance. In an attempt to increase the digestibility of diet supplements have been used with exogenous enzymes to animals. Enzymes are classified according to the role of substrates of enzymes that act on fiber (xylanases and cellulases), protein (proteases), starch (amylases) and phytate (phytases). Chemically, enzymes are proteins with complex three-dimensional molecular structure that has important implications for their stability when exposed to high temperatures or during transit in the digestive tract. Currently, studies with exogenous enzymes in ruminants have focused on the use of enzymes that degrade starch and fiber, thus increasing the digestible energy intake. Some benefits have been observed with the supplementation of exogenous enzymes, such as: degradation of anti-nutritional factors, increased production efficiency, reduce costs and complement the activity of endogenous enzymes. The mechanism of action of exogenous enzymes added to the diets of ruminants is not fully known, and research has focused on the evaluation of each enzyme class individually. The objective of this study is to evaluate the association of Fibrolytic enzymes and amylolytic enzymes on performance and metabolism of dairy cows. Two experiments will be conducted, and the first (experiment 1) will be used 48 dairy cows in an continuous trial (randomized block design), in order to measure consumer variables and nutrient digestion, blood metabolites and performance. In experiment 2, eight dayri cows will be used in a desing in Latin square 4x4, which in addition to measuring the variables already mentioned in the first experiment, also will be assessed ruminal fermentation parameters. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ZILIO, ELISSANDRA M. C.; DEL VALLE, TIAGO A.; GHIZZI, LUCAS G.; TAKIYA, CAIO S.; DIAS, MAURO S. S.; NUNES, ALANNE T.; SILVA, GUILHERME G.; RENNO, FRANCISCO P.. Effects of exogenous fibrolytic and amylolytic enzymes on ruminal fermentation and performance of mid-lactation dairy cows. JOURNAL OF DAIRY SCIENCE, v. 102, n. 5, p. 4179-4189, . (16/15711-8)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
ZILIO, Elissandra Maiara de Castro. Exogenous fibrolytic and amylolytic enzymes at feeding dairy cows. 2018. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) Pirassununga.

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