Screening of fungi phytases produced by solid state fermentation with different agricultural by-products as substrate

Author(s)

Paula Kern Novelli

• Document type: Doctoral Thesis
• Press: Botucatu. 2015-12-10.
• Institution: Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu
• Defense date: 2015-07-31
• Advisor: Luciana Francisco Fleuri; Margarida Maria Barros

Abstract

This study was carried out for screening the phytase production using agroindustrial residues as substrate for solid state fermentation (SSF) and several fungal strains. High phytase production was observed for most of the microorganisms studied, as well as very different biochemical characteristics, including activities at specific pH values. The phytases showed very distinct behavior of optimum and stability pH, optimal values of temperature and stability. A. niger showed the higher values of enzyme activity. Lyophilized enzyme produced by A. niger reached 8,575 U/g and yield of 826,096.50 U/Kg of substrate when fermented in wheat bran. Therefore, the substrate as well as the microorganism strain can affect the biochemical character of the enzyme produced. The high phytase activity and very distinct characteristics established, plus the low cost of substrates, make these fungal phytases potential alternatives for biotechnological industry. (AU)

• FAPESP's process: 13/02998-9 - Production of fungal phytases and proteases, feed application and digestible values for Nile tilapia
• Grantee: Paula Kern Novelli
• Support Opportunities: Scholarships in Brazil - Doctorate