Construction and characterization of double mutant of Salmonella Typhimurium for DNA binding proteins

• Grant number: 14/11280-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2014
• End date: July 31, 2015
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Marcelo Brocchi
• Grantee: Juliana Pastorello Pereira
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Salmonella is a gram-negative bacilli, facultative anaerobic and the Salmonella enterica Typhimurium (S. typhimurium) is the most common and studied serovar that infect humans. This bacterium can be easily genetically manipulated and has an available genome sequence. That makes it possible to use these mutants for different goals, like vaccines and tumor-treatment, considering recent studies using these attenuated strains in an attempt to reduce tumor mass in murine models. This project aims to construct and characterize a double mutant Salmonella Typhimurium from two single mutants previously built by our group. This new mutant can participate in the current studies from the group with the intention of constructing a vaccine strain.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TOMIYAMA, M. P. O.; WERLE, C. H.; MILANEZ, G. P.; NOBREGA, D. B.; PEREIRA, J. P.; CALARGA, A. P.; FLORES, F.; BROCCHI, M.. Salmonella enterica Typhimurium fljBA operon stability: implications regarding the origin of Salmonella enterica I 4,[5], 12:i:-. Genetics and Molecular Research, v. 14, n. 4, p. 19057-19065, 2015. (09/15956-7, 14/11280-7, 13/11880-1, 12/10608-3, 12/05382-6)