Interactions between the arbuscular mycorrhizal fungi interaction and microbial community in soil with sugarcane

• Grant number: 14/11095-5
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: November 01, 2014
• End date: October 31, 2015
• Field of knowledge: Agronomical Sciences - Agronomy - Soil Science
• Principal Investigator: Fernando Dini Andreote
• Grantee: Dorotéia Alves Ferreira
• Supervisor: Joana Falcão Salles
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Institution abroad: University of Groningen, Netherlands
• Associated to the scholarship: 13/04388-3 - INTERACTIONS BETWEEN THE ARBUSCULAR MYCORRHIZAL FUNGI INTERACTION AND MICROBIAL COMMUNITY IN SOIL WITH SUGARCANE, BP.DR

Abstract

Brazil is the largest producer of sugarcane and the state of São Paulo stands out as a major national producer. The sugarcane assumes significant importance for energy, food, environmental and socioeconomic aspects in the country. Several factors contribute to the successful cultivation of this crop, among with it is important to consider the climatic characteristics and microbiological interactions occurring in the soil, providing greater sustainability of production due to maintenance of various biogeochemical processes. However, little is known about the microbial community of soil cultivated with sugarcane and its interactions with important microorganisms for ecosystem function, such as arbuscular mycorrhizal fungi (AMF). This project has the main purpose the understanding of the dynamics that occur on the interaction between sugarcane and AMF species when soil microbial communities present distinct levels of biodiversity. It is proposed to evaluate three AMF species (Glomus clarum, Acaulospora sp. and Scutellospora sp.) in different situations diversity of the microbial community (microbial diversity of natural soil, sterile soil, and three intermediate levels of diversity), established by inoculating dilutions of the extract natural soil (10-3, 10-6 and 10-9) in sterile soil. To analyses will consider rates of sugarcane mycorrhizal colonization, diversity of bacteria and fungi in each treatment and the dry matter of the shoot of plants. The profiles of the microbial communities will be determined by extracting the soil DNA, and further analysis by length polymorphism terminal restriction fragments (T-RFLP). It is intended to correlate the data obtained from surveys, with those observed in areas of sugarcane cultivation, to observe the microbial community structure and the mechanisms that result in the establishment of major bacterial groups and AMF. In addition it will allow to observe bacterial groups which interact with the AMF species studied, and its role large scale cultivation fields. (AU)