Construction of a combined attenuated Salmonella enterica Typhimurium vaccine expressing interleukin 6 (IL-6) carrier of DNA vaccine expressing the S1 protein of infectious bronquitis virus (IBV)

• Grant number: 13/01144-6
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: August 01, 2014
• End date: June 30, 2018
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Marcelo Brocchi
• Grantee: Luciana Helena Antoniassi da Silva
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated scholarship(s): 16/09894-2 - Characterization in vivo of null mutants ihfA and ”ihfAB of Salmonella enterica Typhimurium, BE.EP.PD

Abstract

The genus Salmonella is a member of the Enterobacteriaceae, comprising gram-negative, facultative anaerobes and usually flagellates. Most cases of food poisoning in humans, and infection associated with poultry products such as meat and eggs.Cytokines are regulators of the immune response and are being used as an alternative to conventional therapy against disease, showing efficacy in various studies involving humans and animals. Interleukin 6 (IL-6) influenza is a proinflammatory cytokine, has the same role in mammals and is produced initially after infection as part of the innate immune response.The infectious bronchitis virus (IBV) is one of the most important among the etiologic agents of disease in the poultry industry and may be involved in respiratory disease, nephritis, enteritis, fertility problems and reducing the production and quality of eggs. The VBI encoding four structural proteins: a spike protein (S), a membrane protein (M), the nucleocapsid (N) and the small membrane protein (E). The S protein is divided into two areas (S1 and S2), and that this protein is responsible for binding of the virion to the cell receptors, induces the fusion of the envelope with the plasma membrane and has important antigenic sites which induce neutralizing antibodies and cellular immune response.In this project we intend to further studies to better characterize strains of S. ihfA and ihfB enterica Typhimurium. Such strains are also exploited as carrier DNA vaccine encoding the S protein gene of a Brazilian strain IBV. Furthermore, these strains are engineered to express IL-6 bird in order to increase their immunogenic potential. (AU)