Monitoring of interaction between plant growth promoter rhizobacterium and rhizobium in soybeans [Glycine max (L.) Merr.]

• Grant number: 14/05420-0
• Support type: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): August 01, 2014
• Effective date (End): July 31, 2015
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal researcher: Maria Carolina Quecine Verdi
• Grantee: Andréa Ferrari
• Home Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil

Abstract

Soybean is one of the most important crops in Brazil. The Brazilian production in 2014 probably will reach a record amount of 89.1 million tons of harvest grains. Certainly, the success of soybean production in Brazil is due to the massive application of rhizobia, symbiote bacteria capable of biological nitrogen fixation, converting the atmospheric nitrogen to easy assimilating nitrogen compounds to the host. Some researchers have been proved that the soybean-rhizobia interaction should be optimized by the plant-growth-promoting rhizobacteria (PGPRs) co-inoculation. However, studies about the interaction of rhizobia and PGPRs are incipient in Brazil. Thus, the present project aims the monitoring of the interaction between Burkholderia ambifaria RZ2MS16, a rhizobacterium previously described as a soybean growth promoter, and rhizobia strain commercially used on the crop. All strains will be targeted with fluorescent proteins by transformation. The bacterial interaction will be observed by microscopy of fluorescence as well as by re-isolation of target- strains during the soybean infection. The synergistic effects of this interaction will be also evaluated under green-house condition co-inoculations, being measured the nodules amount as well the improvement of soybean dried weight. The obtained results will certainly corroborate with the better understanding of rhizobia-rhizobacteria interaction as co-agents of soybean growth promoters. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BATISTA, BRUNA DURANTE; LACAVA, PAULO TEIXEIRA; FERRARI, ANDREA; TEIXEIRA-SILVA, NATALIA SOUSA; BONATELLI, MARIA LETICIA; TSUI, SARINA; MONDIN, MATEUS; KITAJIMA, ELLIOT WATANABE; PEREIRA, JOSE ODAIR; AZEVEDO, JOAO LUDO; et al. Screening of tropically derived, multi-trait plant growth- promoting rhizobacteria and evaluation of corn and soybean colonization ability. MICROBIOLOGICAL RESEARCH, v. 206, p. 33-42, 2018. (11/05204-8, 14/05420-0, 10/14382-4, 15/01188-9, 10/15192-4)