Effect of the treatment with the paladacycle complex DPPE 1.2 associated to the recombinant cysteine proteinase rLdccys1 on Leishmania (Leishmania) amazonensis infection in a murine model

• Grant number: 14/06935-4
• Support Opportunities: Scholarships in Brazil - Master
• Start date: July 01, 2014
• End date: February 29, 2016
• Field of knowledge: Biological Sciences - Parasitology - Protozoology of Parasites
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Clara Lúcia Barbiéri Mestriner
• Grantee: Danielle Aparecida Marino da Silva
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

Previous results showed the in vitro and in vivo activity of the palladacycle complex DPPE 1.2 against Leishmania (Leishmania) amazonensis. The treatment of L. (L.) amazonensis-infected BALB/c mice with DPPE 1.2 resulted in a significant decrease of lesion size and parasite load compared to those of untreated animals and no toxicity was observed in treated animals at the concentrations of DPPE 1.2 used. There was an increase of CD4+ and CD8+ T lymphocyte expression in the popliteal and inguinal lymph nodes of mice treated with DPPE 1.2, indicating that DPPE 1.2 modulated the immune system in treated mice. Our previous data also demonstrated that the immunization of BALB/c mice with a recombinant cysteine proteinase from L. (L.) chagasi, rLdccys1, elicited protective immune responses against homologous infection mediated by Th1 lymphocytes. In addition, the cross reactivity between rLdccys1 and a cysteine proteinase of 30 kDa from L. (L.) amazonensis was also demonstrated. These data led us to study the effect of the treatment with DPPE 1.2 associated to rLdccys1 on L. (L.) amazonensis-infected BALB/c mice. The perspective is to obtain a synergistic effect leading to an amplification of the immune responses as well as of the leishmanicidal action of DPPE 1.2 in treated animals. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DOS SANTOS, ISABELA B.; DA SILVA, DANIELLE A. M.; PAZ, FABIANA A. C. R.; GARCIA, DANIEL M.; CARMONA, ADRIANA K.; TEIXEIRA, DANIELA; LONGO-MAUGERI, IEDA M.; KATZ, SIMONE; BARBIERI, CLARA L.. Leishmanicidal and Immunomodulatory Activities of the Palladacycle Complex DPPE 1.1, a Potential Candidate for Treatment of Cutaneous Leishmaniasis. FRONTIERS IN MICROBIOLOGY, v. 9, JUL 3 2018. (14/06935-4, 13/02133-8)

PALADI, CAROLINA S.; DA SILVA, DANIELLE A. M.; MOTTA, PRISCILA D.; GARCIA, DANIEL M.; TEIXEIRA, DANIELA; LONGO-MAUGERI, IEDA M.; KATZ, SIMONE; BARBIERI, CLARA L.. Treatment of Leishmania (Leishmania) Amazonensis-Infected Mice with a Combination of a Palladacycle Complex and Heat-Killed Propionibacterium acnes Triggers Protective Cellular Immune Responses. FRONTIERS IN MICROBIOLOGY, v. 8, MAR 6 2017. (13/02133-8, 14/06935-4, 09/10809-6)