Evaluation of CE-MS for the urinary targeted metabolomics assessment of vesicoureteral reflux

• Grant number: 14/18789-2
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: October 20, 2014
• End date: October 19, 2015
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Marina Franco Maggi Tavares
• Grantee: Karina Trevisan Rodrigues
• Supervisor: Van Schepdael Ann
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Institution abroad: University of Leuven, Leuven (KU Leuven), Belgium
• Associated to the scholarship: 12/09822-0 - Evaluation of urinary global fingerprint of vesicoureteral reflux for CE-MS, BP.DR

Abstract

This project intends to develop and to validate CE-MS methods, at both positive and negative electrospray ionization modes, to explore their capability for the urinary targeted metabolic profiling of amino acids and carboxylic acids in the assessment of Vesicoureteral Reflux. In the method development, compositions of the background electrolyte, sheath liquid and instrumental parameters will be systematically varied and optimized at both ionization modes. Data will be processed and statistically evaluated by means of multivariate methods, including principal component analysis (PCA) and partial least squares projection to latent structures (PLS) based on variants. Discriminatory metabolites will then be quantified by the validated proposed methods. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RODRIGUES, KARINA T.; MEKAHLI, DJALILA; TAVARES, MARINA F. M.; VAN SCHEPDAEL, ANN. Development and validation of a CE-MS method for the targeted assessment of amino acids in urine. ELECTROPHORESIS, v. 37, n. 7-8, SI, p. 1039-1047, APR 2016. (12/09822-0, 14/18789-2)

RODRIGUES, KARINA T.; MEKAHLI, DJALILA; TAVARES, MARINA F. M.; VAN SCHEPDAEL, ANN. Development and validation of a CE-MS method for the targeted assessment of amino acids in urine. ELECTROPHORESIS, v. 37, n. 7-8, p. 9-pg., 2016-04-01. (12/09822-0, 14/18789-2)