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Soil microbiome in different environments and protected areas, with Atlantic Forest and Cerrado remnants: a metagenomic approach

Grant number: 14/14234-6
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): September 01, 2014
Effective date (End): February 29, 2016
Field of knowledge:Agronomical Sciences - Agronomy
Cooperation agreement: Vale-FAPEMIG-FAPESPA
Principal Investigator:Eliana Gertrudes de Macedo Lemos
Grantee:Luciano Takeshi Kishi
Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
Company:Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Faculdade de Ciências Agrárias e Veterinárias (FCAV)

Abstract

Prospection, selection, and enzymatic characterization of superoxide dismutase and catalase in metagenomic libraries from different soils: The intense industrial progress observed currently generates large amounts of pollutants that are harmful to the environment. These residues cause irreversible impacts to the sites where it is introduced and it is common the use of organisms as biomarkers for these polluted sites. The relationship between the enzymatic activity and the effects caused by the stressor in living systems causes the production of reactive oxygen species (ROS) such as superoxide ion (O2-), hydrogen peroxide (H2O2), hydroxyl radical (OH-) and single free oxygen (O1). These compounds can cause cell death if not neutralized and, therefore, the aerobic organisms have developed a defense mechanism against oxidative stress and also the synthesis of superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR) and glutathione S-transferase (GST). These biomolecules are used in industrial processes and presented the advantage of high specificity to the substrate in relation to chemicals, significantly reducing the production of products in undesirable reaction and purification steps, and presents the possibility of immobilization and recovery of these enzymes for new uses in the process, not requiring high temperatures and pressures in different operations. In general, these biocatalysts are obtained at different scales (laboratory, pilot or industrial) making the use of recombinant DNA technology from the expression of homologous or heterologous genes of biotechnological interest. The oxido-reductases such as SOD and CAT are used in the same way that the hydrolytic enzymes, however, have enormous potential for use as the trend in increasing market share, as in the medical, analytical and scientific, clinical diagnostics, food industry in the formulation of cosmetics, and in bioremediation process of effluents containing phenolic compounds and aromatic amines. In this context, the tool called metagenomics has been widely used to obtain enzymes of biotechnological interest, mainly because this approach will allow access to genes from uncultivable microorganisms, since the analysis involving the study of microbial diversity showed that we know just 1% presented in biosphere. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FERNANDES, CAMILA CESARIO; KISHI, LUCIANO TAKESHI; LOPES, ERICA MENDES; OMORI, WELLINGTON PINE; MARCONDES DE SOUZA, JACKSON ANTONIO; CARARETO ALUES, LUCIA MARIA; DE MACEDO LEMOS, ELIANA GERTRUDES. Bacterial communities in mining soils and surrounding areas under regeneration process in a former ore mine. Brazilian Journal of Microbiology, v. 49, n. 3, p. 489-502, JUL-SEP 2018. Web of Science Citations: 0.
KISHI, LUCIANO TAKESHI; FERNANDES, CAMILA CESARIO; OMORI, WELLINGTON PINE; CAMPANHARO, JOAO CARLOS; DE MACEDO LEMOS, ELIANA GERTRUDES. Reclassification of the taxonomic status of SEMIA3007 isolated in Mexico B-11A Mex as Rhizobium leguminosarum bv. viceae by bioinformatic tools. BMC Microbiology, v. 16, NOV 4 2016. Web of Science Citations: 2.

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