Panel detection human herpesvirus by nested polymerase chain reaction in patients with Systemic Lupus Erythematosus

• Grant number: 14/11430-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2014
• End date: August 31, 2015
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Sandra Helena Alves Bonon
• Grantee: Alex Domingos Reis
• Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Human herpesvirus (HHV), including human herpesvirus simplex types 1 and 2 (HSV-1 and HSV-2), human herpesvirus 3 (Varicella Zoster Virus - VZV), human herpesvirus 4 (Epstein-Barr virus - EBV), human herpesvirus 5 (Cytomegalovirus - CMV), human herpesvirus 6 (HHV-6), human herpesvirus 7 (HHV-7) and human herpesvirus 8(HHV-8) are members of the Herpesviridae family. Often, can cause serious complications in patients with autoimmune diseases. HHV are important pathogens and are linked to cases of infections. To check the pathogenesis and prevalence of infections caused by HHV in SLE patients and to investigate the presence of coinfection among these viruses, will be used methods of molecular biology such as Nested Polymerase Chain Reaction (Nested-PCR) with primers specific to each virus researched. The use of techniques for detection of viral DNA may be useful because few studies have evaluated the impact of these infections in the care of severe autoimmune diseases, such as SLE. This work will be of fundamental importance to contribute to this group of patients in order to provide greater knowledge and greater attention and care to individuals affected by SLE and infections by HHV.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DOS REIS, ALEX DOMINGOS; MUDINUTTI, CRISTIANE; DE FREITAS PEIGO, MURILO; LEON, LUCAS LOPES; COSTALLAT, LILIAN TEREZA LAVRAS; ROSSI, CLAUDIO LUCIO; COSTA, SANDRA CECILIA BOTELHO; BONON, SANDRA HELENA ALVES. Active human herpesvirus infections in adults with systemic lupus erythematosus and correlation with the SLEDAI score. ADVANCES IN RHEUMATOLOGY, v. 60, n. 1, p. 7-pg., 2020-08-24. (14/11430-9)

ALEX DOMINGOS DOS REIS; CRISTIANE MUDINUTTI; MURILO DE FREITAS PEIGO; LUCAS LOPES LEON; LILIAN TEREZA LAVRAS COSTALLAT; CLAUDIO LUCIO ROSSI; SANDRA CECÍLIA BOTELHO COSTA; SANDRA HELENA ALVES BONON. Active human herpesvirus infections in adults with systemic lupus erythematosus and correlation with the SLEDAI score. ADVANCES IN RHEUMATOLOGY, v. 60, 2020-09-04. (14/11430-9)