Identification of protein markers changed by shear stress

• Grant number: 13/13526-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: August 01, 2014
• End date: July 31, 2018
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Alexandre da Costa Pereira
• Grantee: Gabriela Venturini da Silva
• Host Institution: Instituto do Coração Professor Euryclides de Jesus Zerbini (INCOR). Hospital das Clínicas da Faculdade de Medicina da USP (HCFMUSP). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
• Associated scholarship(s): 16/02406-2 - Identification of proteins markers of endothelial shear stress intensity, BE.EP.DR

Abstract

Endothelial cells play an important role in the cardiovascular system both physical manner, acting as a non-thrombogenic barrier and selective permeability of macromolecules and circulating cells from blood flow and vascular wall or biochemistry manner, acting as a signaling of cell migration, remodeling, apoptosis, production, secretion and metabolism of biomolecules, beyond regulating the contractility of smooth muscle cells. These cells can be chemically modulated by cytokines and chemokines or physically by hemodynamic forces of the blood flow as the shear stress (SS). The SS is a physical stimulus caused by the traction force of the blood parallel to the endothelium, being able to change the profile protein, secretory and intracellular and extracellular signaling. Changes in SS is highly correlated with the formation and progression of atherosclerotic plaques. Vascular regions that have reduced and altered SS from laminar to oscillatory, such as bifurcations, there is an increased endothelial permeability, alterations in the cytoskeleton and increased expression of adhesion molecules on the membrane of endothelial cells. Although very is explored the relationship between SS and atherosclerotic plaques, a few is known about the signaling pathways of phosphorylation and changes involved in this process, and how this is associated with plaque vulnerability. This work aims identify proteins differently phosphorylated in response to changes in SS through proteomics and metabolic labeling technique. The target proteins will be validated by immunohistochemistry at different levels and atherosclerotic plaques associated with the degree of vulnerability.

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MALAGRINO, PAMELLA ARAUJO; VENTURINI, GABRIELA; YOGI, PATRICIA SCHNEIDER; DARIOLLI, RAFAEL; PADILHA, KALLYANDRA; KIERS, BIANCA; GOIS, TAMIRIS CARNEIRO; MORAIS CARDOZO, KARINA HELENA; CARVALHO, VALDEMIR MELECHCO; SALGUEIRO, JESSICA SILVA; et al. Proteome analysis of acute kidney injury - Discovery of new predominantly renal candidates for biomarker of kidney disease. JOURNAL OF PROTEOMICS, v. 151, n. SI, p. 66-73, JAN 16 2017. (12/05447-0, 13/13526-0, 13/17368-0, 12/12042-7, 11/04344-0)

MALAGRINO, PAMELLA ARAUJO; VENTURINI, GABRIELA; YOGI, PATRICIA SCHNEIDER; DARIOLLI, RAFAEL; PADILHA, KALLYANDRA; KIERS, BIANCA; GOIS, TAMIRIS CARNEIRO; MORAIS CARDOZO, KARINA HELENA; CARVALHO, VALDEMIR MELECHCO; SALGUEIRO, JESSICA SILVA; et al. Proteome analysis of acute kidney injury - Discovery of new predominantly renal candidates for biomarker of kidney disease. JOURNAL OF PROTEOMICS, v. 151, p. 8-pg., 2017-01-16. (12/05447-0, 13/13526-0, 11/04344-0, 13/17368-0, 12/12042-7)

VENTURINI, GABRIELA; MALAGRINO, PAMELLA ARAUJO; PADILHA, KALLYANDRA; TANAKA, LEONARDO YUJI; LAURINDO, FRANCISCO RAFAEL; DARIOLLI, RAFAEL; CARVALHO, VALDEMIR MELECHCO; MORAIS CARDOZO, KARINA HELENA; KRIEGER, JOSE EDUARDO; PEREIRA, ALEXANDRE DA COSTA. Integrated proteomics and metabolomics analysis reveals differential lipid metabolism in human umbilical vein endothelial cells under high and low shear stress. AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, v. 317, n. 2, p. C326-C338, AUG 2019. (16/02406-2, 13/13526-0, 13/17368-0)

MALAGRINO, PAMELLA ARAUJO; VENTURINI, GABRIELA; YOGI, PATRICIA SCHNEIDER; DARIOLLI, RAFAEL; PADILHA, KALLYANDRA; KIERS, BIANCA; GOIS, TAMIRIS CARNEIRO; MOTTA-LEAL-FILHO, JOAQUIM MAURICIO; TAKIMURA, CELSO KIYOCHI; COSTA GIRARDI, ADRIANA CASTELLO; et al. Metabolomic characterization of renal ischemia and reperfusion in a swine model. Life Sciences, v. 156, p. 57-67, JUL 1 2016. (12/05447-0, 13/13526-0, 13/17368-0, 12/12042-7, 11/04344-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SILVA, Gabriela Venturini da. Identification of proteic biomarkers of low and high shear stress. 2018. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina (FM/SBD) São Paulo.