Is the nucleotide excision repair pathway involved in the mechanism of action of urban air pollutants?

• Grant number: 14/02297-3
• Support Opportunities: Scholarships in Brazil - Post-Doctorate
• Effective date (Start): September 01, 2014
• Effective date (End): December 31, 2016
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Paulo Hilário Nascimento Saldiva
• Grantee: Nilmara de Oliveira Alves Brito
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Atmospheric pollution is an environmental risk factor in large urban centers and, currently, is associated with mortality from lung cancer. In this context, the São Paulo Metropolitan Area stands out, due to its important industrial complex and, with seven million vehicles, possesses one the largest fleet worldwide. Research shows that exposure to air pollutants can cause various health effects such as: increased pro- inflammatory response, DNA damage, oxidative stress and others. Such effects induce various other cellular responses, such as the formation of DNA damage,that can cause a mutagenic event if not efficiently removed by various DNA repair pathways. To date, there are few studies focusing on the results from the mechanism of action of urban air pollutants testing the nucleotide excision repair pathway. Therefore, the detailed project proposes investigate the mechanism of action of the particulate matter (PM), from the Sao Paulo Metropolitan Area, associated with DNA damage and if this damage can lead to formation of neoplasms, especially in the lung. For this project, mice deficient in this repair pathway, KO for XPA gene will be employed. The results of this study shall support knowledge of the mechanisms of action of pollutants associated with lung cancer. Such study will be supported by quantification of nanoparticles and chemical characterization of airborne pollutants in the São Paulo Metropolitan Area. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALVES, NILMARA DE OLIVEIRA; VESSONI, ALEXANDRE TEIXEIRA; QUINET, ANNABEL; FORTUNATO, RODRIGO SOARES; KAJITANI, GUSTAVO SATORU; PEIXOTO, MILENA SIMOES; HACON, SANDRA DE SOUZA; ARTAXO, PAULO; SALDIVA, PAULO; MARTINS MENCK, CARLOS FREDERICO; et al. Biomass burning in the Amazon region causes DNA damage and cell death in human lung cells. SCIENTIFIC REPORTS, v. 7, SEP 7 2017. (14/15982-6, 14/02297-3, 13/05014-0)