Analysis of the environmental effects of Kraft and score pesticides and its active ingredients abamectin and difenoconazole in tropical surface waters by biomarkers

• Grant number: 14/14621-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: October 01, 2014
• End date: June 30, 2018
• Field of knowledge: Biological Sciences - Biochemistry - Metabolism and Bioenergetics
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Evaldo Luiz Gaeta Espindola
• Grantee: Ana Letícia Madeira Sanches
• Host Institution: Escola de Engenharia de São Carlos (EESC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Associated scholarship(s): 17/05543-3 - Comparative study of the abamectin and difenoconazole toxic effects in embryos and adults of Danio rerio, BE.EP.DR

Abstract

The fate of pesticides applied on tropical crops is mainly influenced by high rates of rainfall and the runoff. The occurrence of runoff after applications of pesticides affects the dissipation and toxicity of these compounds to non-target organisms in the soil, and also those that make up the biological structure of aquatic environments. Many pesticides are used in agriculture, especially abamectin (Kraft) and difenoconazole (Score), which are heavily used compounds in strawberry and potato crops in tropical regions, although these compounds are classified as extremely toxic and very dangerous to the environment. Considering the ecological risks in the use of these pesticides, the main objective of this research is to evaluate the environmental effects of pesticides Kraft and Score and its active components, abamectin and difenoconazole in tropical agroecosystems, considering runoff. Toxicological effects on fish (Danio rerio) will be analyzed by laboratory and in situ (mesocosms) experiments. In the analysis of environmental effects will be assessed, in addition to mortality of the test organisms, biomarkers of aquatic contamination related to the activities of biotransformation enzymes (glutathione-s-transferase, and glucuronosyltransferase-7-O-etóxiresorufina deetilase), antioxidant defense (glutathione peroxidase, catalase and superoxide dismutase) and parameters indicative of oxidative stress (lipid hydroperoxide, levels of malondialdehyde and protein carbonyl). Analytical methods for determination of abamectin and difenoconazole will be developed and further validation will be used to quantify the concentrations of the water and sediment matrices. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MADEIRA SANCHES, ANA LETICIA; DAAM, MICHIEL ADRIAAN; FREITAS, EMANUELA CRISTINA; GODOY, ALINE ANDRADE; MEIRELES, GABRIELA; ALMEIDA, ANA RITA; DOMINGUES, INES; GAETA ESPINDOLA, EVALDO LUIZ. Lethal and sublethal toxicity of abamectin and difenoconazole (individually and in mixture) to early life stages of zebrafish. Chemosphere, v. 210, p. 531-538, NOV 2018. (17/05543-3, 14/14621-0)

MEIRELES, GABRIELA; DAAM, MICHIEL A.; MADEIRA SANCHES, ANA LETICIA; ZANONI, MARIA V. B.; SOARES, AMADEU M. V. M.; GRAVATO, CARLOS; DE OLIVEIRA, DANIELLE P.. Red disperse dyes (DR 60, DR 73 and DR 78) at environmentally realistic concentrations impact biochemical profile of early life stages of zebrafish (Danio rerio). Chemico-Biological Interactions, v. 292, p. 94-100, AUG 25 2018. (17/05543-3, 14/14621-0, 16/03281-9)

MADEIRA SANCHES, ANA LETICIA; VIEIRA, BRUNA HORVATH; REGHINI, MARINA VANDERLEI; MOREIRA, RAQUEL APARECIDA; FREITAS, EMANUELA CRISTINA; ESPINDOLA, EVALDO L. G.; DAAM, MICHIEL A.. Single and mixture toxicity of abamectin and difenoconazole to adult zebrafish (Danio rerio). Chemosphere, v. 188, p. 582-587, DEC 2017. (14/14621-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SANCHES, Ana Letícia Madeira. Analysis of the environmental effect of the insecticide Kraft 36EC® (abamectin) and the fungicide Score 250EC® (difenoconazole) by means of ecotoxicological analyzes in different Danio rerio life stages. 2018. Doctoral Thesis - Universidade de São Paulo (USP). Escola de Engenharia de São Carlos (EESC/SBD) São Carlos.