Ag/²-calcium glycerophosphate nanocomposite: synthesis, and effect on Candida albicans and Streptococcus mutans biofilm and on odontoblasts MDPC-23 cells

• Grant number: 14/08648-2
• Support Opportunities: Scholarships in Brazil - Master
• Start date: October 01, 2014
• End date: February 29, 2016
• Field of knowledge: Health Sciences - Dentistry
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Debora Barros Barbosa
• Grantee: Gabriela Lopes Fernandes
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

This study aims to syntheze and characterize a new nanocomposite based on silver nanoparticles and ²-calcium glycerophosphate nanoparticles (Ag/ ²-Calcium glycerophosphate) by different routes (chemical and green), and to evaluate their antimicrobial efficacy against Candida albicans (324LA 94) and Streptococcus mutans (ATCC 35668) and the action this nanocomposite on fibroblasts (L929). The chemical synthesis has been performed by two different reducing agents of silver nitrate (sodium borihidreto and sodium citrate) which are associated with calcium ²-glycerophosphate nanoparticulate previously nanoparticulate by grinding process. For the green synthesis will be used peel extract of Punica granatum (pomegranate) obtained by methanol and ethanol extraction. These extracts have been analyzed physical, chemical and pharmacologically and characterized by HPLC (having as primary standard ellagic acid). These extracts are then individually added to silver nitrate, sodium citrate, ²-Calcium glycerophosphate. The nanocomposites obtained by chemical and green routes have concentrations of 1 and 10% silver. The summaries will be monitored by UV-Visible, X-ray diffraction, scanning electron microscopy. Then, the minimum inhibitory concentration of each nanocomposite able to inhibit the growth of C. albicans and S. mutans in the planktonic (MIC) and sessile state (MBC) will be determined. Compounds that produce effects against these microorganisms are screened for cytotoxicity testing in primary cultures of fibroblast cells (L929) where the proliferation will be evaluated by MTT. All tests, microbiological and cellular toxicity, will be performed in triplicate on three different occasions. Data will be analyzed to employ the most appropriate statistical test. P< .05 will be considered statistically significant. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERNANDES, GABRIELA LOPES; BOTAZZO DELBEM, ALBERTO CARLOS; DO AMARAL, JACKELINE GALLO; GORUP, LUIZ FERNANDO; FERNANDES, RENAN APARECIDO; DE SOUZA NETO, FRANCISCO NUNES; SANTOS SOUZA, JOSE ANTONIO; MONTEIRO, DOUGLAS ROBERTO; AGOSTINHO HUNT, ALESSANDRA MARCAL; CAMARGO, EMERSON RODRIGUES; et al. Nanosynthesis of Silver-Calcium Glycerophosphate: Promising Association against Oral Pathogens. ANTIBIOTICS-BASEL, v. 7, n. 3, SEP 2018. (14/08648-2, 13/24200-9)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

FERNANDES, Gabriela Lopes. Silver nanoparticles and calcium glycerophosphate: synthesis by conventional and phytochemical routes, antimicrobial analysis and evaluation of cytotoxicity.. 2016. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araçatuba Araçatuba.