Silver nanoparticles associated with sodium hexamehtaphosphate and fluoride: synthesis and antimicrobial effect

• Grant number: 16/17935-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: December 01, 2016
• End date: November 30, 2017
• Field of knowledge: Health Sciences - Dentistry - Dental Materials
• Principal Investigator: Debora Barros Barbosa
• Grantee: Willian Henrique Jacometo
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

The aim of this study will be to synthesize a nanocompound contaning silver nanoparticles (Ag-NP), sodium hexamethaphosphate (HMP) and fluoride (F), and charactherize and evaluate its antimicrobial action against planktonic cells of Candida albicans and Streptococcus mutans. Ag-NP will be synthesized by reducing the silver ions from silver nitrate by a chemical route and in the presence of F and HMP. This compound will be characterized by scanning eletron micrography (SEM), X-ray difraction (EDX) and UV/Vis spectroscopy (UV/Vis), and silver ions wil lbe quantified by a specific electrode-ion. Microdilution method will be carried out to evaluate the minimum inhibitory concentration (MIC) effective against Streptococcus mutans (ATCC 25175) e Candida albicans ATCC 10231. This assay will follow the Clinical Laboratory Standards Institute (CLSI) standards for each strain. After the compound dilution in the microroganism suspension, the the microtite plates will be incubated at 37ºC for 24 hours in microaerophilia and 48 hours in aerophilia, respectively for Streptococccus mutans and Candida albicans. The MICs will be determined as being the the lowest concentration which will inhibite the microorganism growth. The assays will be carried out in triplicate in three different occasions.