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Effect of phenolic compounds from the fruit camu-camu (Myrciaria dubia HBK McVaugh) in Non-Alcoholic Fatty Liver Disease (NAFLD) in mice

Grant number: 14/14625-5
Support type:Scholarships in Brazil - Master
Effective date (Start): October 01, 2014
Effective date (End): July 31, 2016
Field of knowledge:Agronomical Sciences - Food Science and Technology - Food Science
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal researcher:Maria Ines Genovese Rodriguez
Grantee:Luana Jorge de Sousa
Home Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated research grant:13/50506-8 - Bioactive components from by-products of food processing used in a symbiotic approach for improving human health and well-being (BioSyn), AP.TEM


The rates of obesity and diseases associated with it, such as Non-Alcoholic Fatty Liver Disease (NAFLD), are increasing fast. One of the main ways to decrease the risk of these complications is diet management. In this respect, foods rich in phenolic compounds could play an important role due to their anti-inflammatory properties and improvement of the lipid profile and consequently of liver functioning. Fruit native to the Amazon, camu-camu (Myrciaria dubia HBK McVaugh) is rich in these compounds, which makes it a potential help in improving this clinical picture. The objective of this study is to evaluate the effect of extracts enriched with phenolic compounds from camu-camu in the pathophysiology of NAFLD, from an in vivo experiment with 40 black male mice of C57BL/6 mice. Fed with High Fat diet with High Sucrose (HF/HS), the animals receive phenolic fruit extracts (by gavage), obtained by solid phase extraction, during the period of 8 weeks. The animals will be randomly divided into 4 groups of ten, with group 1 (control) receive standard laboratory chow and water, group 2 will be fed with the HF/HS diet and water, group 3 receive the same HF diet/HS extracts and phenolic compounds camu camu (1 g/kg body weight) and group 4 will HF diet/HS extracts and phenolic compound (2 g/kg body weight). The experimental protocol was approved by the Ethics Committee on Animal Use of the School of Pharmaceutical Sciences, University of São Paulo (CEUA/FCF/353). Food and water intake, body weight, blood glucose, fasting plasma triacylglycerols and after 3 hours of power will be assessed. After euthanasia, will be evaluated serum content of cholesterol, HDL-C, triglyceride, AST/ALT, liver lipid content and lipid dose in feces. Will be collected and weighed the epididymal, retroperitoneal and inguinal adipose tissues and also the heart, gastrocnemius muscle and liver. The liver will be used for subsequent histological analysis, lipid peroxidation, antioxidant capacity, glycogen content and gene expression that decode proteins involved in hepatic lipid metabolism. (AU)

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Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SOUSA, Luana Jorge de. Effect of camu-camu fruit phenolic compounds (Myrciaria dubia H. B. K. Mc Vaugh) on nonalcoholic fatty liver disease (NAFLD) in mice.. 2016. Master's Dissertation - Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ) São Paulo.

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