Identification of tumoral stem cells in head and neck cancer: genetic and protein expression in the response to chemotherapy

• Grant number: 14/15009-6
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: November 01, 2014
• End date: January 31, 2017
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Eny Maria Goloni Bertollo
• Grantee: Ana Lívia Silva Galbiatti-Dias
• Host Institution: Faculdade de Medicina de São José do Rio Preto (FAMERP). Secretaria de Desenvolvimento Econômico (São Paulo - Estado). São José do Rio Preto , SP, Brazil
• Associated scholarship(s): 15/00887-0 - Investigation the response of head and neck cancer stem cells to various therapeutic strategies to overcome chemotherapy resistance, BE.EP.PD

Abstract

Head and neck cancer(CCP) is the fifth most common type of cancer and the survival rate has not changed in recent years. Chemotherapy treatment for the disease has shown unfavorable results due to tumor resistance. This fact is possibly due to the existence of a small subpopulation of cells in these tumors with properties of stem cells, called tumor stem cells (CTT), responsible for sustaining tumor growth by tumor resistance to conventional therapies and recurrence of disease. Therefore, the objectives of the study is to identify the presence of CTT in CCP and cell lines, to verify the effectiveness of chemotherapy in different conditions of exposure time, to analyze mRNA expression of genes related to the presence of CTT and the association of mRNA expression at the level of aggressiveness of the tumor. First the CTT will be identified by flow cytometry, then chemotherapy will be applied. To assess cell viability, MTT technique will be performed to evaluate apoptosis and flow cytometry technique with specific antibodies will be utilized. After, mRNA and protein of CD44, CD133, CD117 and ALDH1 genes will be evaluated by Real Time and ELISA, respectively. All results will be evaluated by appropriate statistical analysis. Studies of clinical response are needed to obtain a combination of molecular parameters prognostic factors. Hopefully, the results of this proposal contribute to new therapeutic strategies with information about estimation of prognosis and treatment decision (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SILVA GALBIATTI-DIAS, ANA LIVIA; MENDONCA FERNANDES, GLAUCIA MARIA; URBANIN CASTANHOLE-NUNES, MARCIA MARIA; HIDALGO, LUIZA FERNANDES; VIESI NASCIMENTO FILHO, CARLOS HENRIQUE; KAWASAKI-OYAMA, ROSA SAYOKO; MUNIZ FERREIRA, LETICIA ANTUNES; BISELLI-CHICOTE, PATRICIA MATTOS; PAVARINO, ERIKA CRISTINA; GOLONI-BERTOLLO, ENY MARIA. Relationship between CD44(high)/CD133(high)/CD117(high) cancer stem cells phenotype and Cetuximab and Paclitaxel treatment response in head and neck cancer cell lines. AMERICAN JOURNAL OF CANCER RESEARCH, v. 8, n. 8, p. 1633-1641, 2018. (15/04403-8, 14/15009-6)

DE MENDONCA FERNANDES, GLAUCIA MARIA; SILVA GALBIATTI-DIAS, ANA LIVIA; MUNIZ FERREIRA, LETICIA ANTUNES; SERAFIM JUNIOR, VILSON; RODRIGUES-FLEMING, GABRIELA HELENA; DE OLIVEIRA-CUCOLO, JULIANA GARCIA; BISELLI-CHICOTE, PATRICIA MATOS; KAWASAKI-OYAMA, ROSA SAYOKO; MANIGLIA, JOSE VICTOR; PAVARINO, ERIKA CRISTINA; et al. Anti-EGFR treatment effects on laryngeal cancer stem cells. AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH, v. 13, n. 1, p. 143-155, 2021. (16/20087-1, 15/04403-8, 14/15009-6)