Using of a mutant in gibberellin in grafting of the tomato to study signaling this hormone between roots and shoots during water deficit

• Grant number: 14/19165-2
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: November 01, 2014
• End date: October 31, 2017
• Field of knowledge: Agronomical Sciences - Agronomy
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Rogério Falleiros Carvalho
• Grantee: Lucas Aparecido Gaion
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

Evidence shows the mobility of gibberellins (GAs) from the roots to the shoots during development, indicating the involvement of this hormone in the responses of shoots to stress conditions, in particular, the most worrying of abiotic stresses drought. Thus, the objective of this study is to evaluate the importance of roots as a source of GA signaling of water stress to the shoot. For this, we propose the technique of grafting a mutant showing high sensitivity to GA (procera or pro) as rootstock and the missing control of the mutation (Micro-Tom or MT). To this end will be used six treatments consisting of the combination between the control and the mutant plant and the controls without grafting to be analyzed the following responses: (I) Analysis of growth; dry weight of roots and shoots, length, area and density of roots, stem elongation, leaf area of shoots and fluorescence; (II) biochemical analyzes; contents of chlorophylls and carotenoids, enzyme activity, peroxidases, hydrogen peroxide (H2O2) and malondealdeído (MDA) as well as quantification of GA in roots and shoots; and (III) molecular analysis of LeDREB2 a gene response to drought stress. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GAION, LUCAS APARECIDO; CARVALHO, ROGERIO FALLEIROS. Long-Distance Signaling: What Grafting has Revealed?. JOURNAL OF PLANT GROWTH REGULATION, v. 37, n. 2, p. 694-704, JUN 2018. (14/19165-2)

GAION, LUCAS A.; CARVALHO, ROGERIO F.. Stomatal response to drought is modulated by gibberellin in tomato. ACTA PHYSIOLOGIAE PLANTARUM, v. 43, n. 9, SEP 2021. (14/19165-2)

GAION, LUCAS APARECIDO; MONTEIRO, CAROLINA CRISTINA; RODRIGUES CRUZ, FLAVIO JOSE; ROSSATTO, DAVI RODRIGO; LOPEZ-DIAZ, ISABEL; CARRERA, ESTHER; LIMA, JONI ESROM; PEREIRA PERES, LAZARO EUSTAQUIO; CARVALHO, ROGERIO FALLEIROS. Constitutive gibberellin response in grafted tomato modulates root-to-shoot signaling under drought stress. Journal of Plant Physiology, v. 221, p. 11-21, FEB 2018. (14/19165-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

GAION, Lucas Aparecido. Use of a gibberellin mutant in grafting of the tomato to study the role of this hormone in the signaling between root and shoot during drought stress. 2017. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.