Cadmium transport and accumulation of different tomato genotypes: physiological, proteomic and molecular approaches.

• Grant number: 16/14349-3
• Support Opportunities: Regular Research Grants
• Duration: December 01, 2016 - November 30, 2018
• Field of knowledge: Agronomical Sciences - Agronomy
• Principal Investigator: Ricardo Antunes de Azevedo
• Grantee: Ricardo Antunes de Azevedo
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated researchers: Berenice Kussumoto de Alcântara da Silva ; Fernanda Salvato ; Fernando Angelo Piotto ; Katherine Derlene Batagin Piotto ; Salete Aparecida Gaziola

Abstract

In the last decades, agriculture has been changed with arising of new scenarios of environmental problems, especially those involving the contamination of soil and water with heavy metals. These problems are more serious when we consider that metals such as cadmium (Cd) can easily enter in food chain by their absorption via the roots of the plant species used as foods. Although several studies have been conducted to understand the Cd impact on oxidative metabolism of crops, there are little information about the kinetics of uptake and accumulation of this metal in the tissues, as well as on dynamic of responses in biochemical and molecular levels throughout the process. This information is extremely important because they can ravel mechanisms response to Cd and mechanisms of tolerance in an integrated and complex system. Then, objective of this work is characterize the physiological, biochemical and molecular changes during the process of absorption and Cd accumulation, through the integrated analysis of all these processes using sensitive tomato genotypes to this metal. We hope to draw a map in which the processes of absorption and Cd accumulation in tomato plants are monitored from the first moment in which the plants come into contact with the metal until the time that they are under chronic stress. Associated with these kinetic studies of absorption and Cd accumulation, we intend to trace the cellular antioxidant system response profile, in addition to conducting the study on protein change, phytochelatin synthesis and molecular level of expression of genes related to the transport of ions on solution to the plant via root tissues. For that, we will apply methodology of analysis of Cd uptake kinetics and nutrients over time, conducting experiments with plants in hydroponics system, to identify the most important times of recognition and response to stress. In addition, we will use reciprocal grafting between tolerant and susceptible tomato genotypes, as tools to study the signaling between the root system and aerial parts of the plants during the process of absorption and metal accumulation in tissues. These results and the previous knowledge of other researches involving the response of crops to Cd exposure, aggregating the experience of our research group, certainly will generate relevant information, which will be published in good refereed international journals that are respected in the field. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

REIS BORGES, KARINA LIMA; SALVATO, FERNANDA; LOZIUK, PHILIP L.; MUDDIMAN, DAVID C.; AZEVEDO, RICARDO ANTUNES. Quantitative proteomic analysis of tomato genotypes with differential cadmium tolerance. Environmental Science and Pollution Research, v. 26, n. 25, p. 26039-26051, SEP 2019. (09/54676-0, 16/14349-3)

CARVALHO, MARCIA E. A.; CASTRO, PAULO R. C.; KOZAK, MARCIN; AZEVEDO, RICARDO A.. The sweet side of misbalanced nutrients in cadmium-stressed plants. Annals of Applied Biology, v. 176, n. 3, JAN 2020. (09/54676-0, 15/26640-1, 16/14349-3, 13/15217-5)

REIS BORGES, KARINA LIMA; SALVATO, FERNANDA; ALCANTARA, BERENICE KUSSUMOTO; NALIN, RAFAEL STORTO; PIOTTO, FERNANDO ANGELO; AZEVEDO, RICARDO ANTUNES. Temporal dynamic responses of roots in contrasting tomato genotypes to cadmium tolerance. ECOTOXICOLOGY, v. 27, n. 3, p. 245-258, APR 2018. (09/54676-0, 16/14349-3)

MARQUES, DEYVID NOVAES; STOLZE, SARA CHRISTINA; HARZEN, ANNE; NOGUEIRA, MARINA LIMA; BATAGIN-PIOTTO, KATHERINE DERLENE; PIOTTO, FERNANDO ANGELO; MASON, CHASE; AZEVEDO, RICARDO ANTUNES; NAKAGAMI, HIROFUMI. omparative phosphoproteomic analysis of tomato genotypes with contrasting cadmium toleranc. Plant Cell Reports, v. 40, n. 10, AUG 2021. (16/05788-3, 20/12666-7, 18/20706-9, 16/14349-3, 17/05544-0)

CARVALHO, MARCIA E. A.; GAZIOLA, SALETE A.; CARVALHO, LILIAN A.; AZEVEDO, RICARDO A.. Cadmium effects on plant reproductive organs: Physiological, productive, evolutionary and ecological aspects. Annals of Applied Biology, v. 178, n. 2, SI, JUN 2020. (13/15217-5, 09/54676-0, 16/14349-3, 15/26640-1)

REIS BORGES, KARINA LIMA; RIEGER HIPPLER, FRANZ WALTER; AMARAL CARVALHO, MARCIA EUGENIA; NALIN, RAFAEL STORTO; MATIAS, FILIPE INACIO; AZEVEDO, RICARDO ANTUNES. Nutritional status and root morphology of tomato under Cd-induced stress: Comparing contrasting genotypes for metal-tolerance. Scientia Horticulturae, v. 246, p. 518-527, FEB 27 2019. (09/54676-0, 15/26640-1, 16/14349-3, 13/15217-5)

MARQUES, DEYVID NOVAES; NOGUEIRA, MARINA LIMA; GAZIOLA, SALETE APARECIDA; BATAGIN-PIOTTO, KATHERINE DERLENE; FREITAS, NATALIA CHAGAS; ALCANTARA, BERENICE KUSSUMOTO; PAIVA, LUCIANO VILELA; MASON, CHASE; PIOTTO, FERNANDO ANGELO; AZEVEDO, RICARDO ANTUNES. New insights into cadmium tolerance and accumulation in tomato: Dissecting root and shoot responses using cross-genotype grafting. Environmental Research, v. 216, p. 14-pg., 2023-01-01. (20/12666-7, 22/11018-7, 17/05544-0, 18/20706-9, 21/06117-3, 16/05788-3, 16/14349-3)

CARVALHO, MARCIA E. A.; GAZIOLA, SALETE A.; CARVALHO, LILIAN A.; AZEVEDO, RICARDO A.. Cadmium effects on plant reproductive organs: Physiological, productive, evolutionary and ecological aspects. Annals of Applied Biology, v. 178, n. 2, p. 17-pg., 2020-06-24. (09/54676-0, 16/14349-3, 13/15217-5, 15/26640-1)