Construction of infectious cDNA clones of encephalitic flaviviruses and identification of molecular determinants in the e glycoprotein for viral infectivity

• Grant number: 14/20930-5
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: March 05, 2015
• End date: March 04, 2016
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Victor Hugo Aquino Quintana
• Grantee: Alberto Anastacio Amarilla Ortiz
• Supervisor: Alexander A. Khromykh
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Institution abroad: University of Queensland, Brisbane (UQ), Australia
• Associated to the scholarship: 12/08778-8 - Analysis of participation the E protein in neuroinvasiveness and neurovirulence of Rocio virus in an animal model, BP.PD

Abstract

Rocio virus (ROCV) was the causative agent of an outbreak of encephalitis occurred in the Ribeira Valley region, Sao Paulo State, Brazil, in 1975, which was described as a new virus of the genus Flavivirus, family Flaviviridea. To study the mechanisms of neurovirulence and neuroinvasiveness of ROCV, the construction of infectious cDNA clone of ROCV and identification of molecular determinants in the E glycoprotein for viral infectivity will be performed. This construction will be done in collaboration with Professor Alexander Khromykh's RNA Virology Laboratory at the University of Queensland, Australia, who has extensive experience in the construction and production of infectious clones of flaviviruses. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

AMARILLA, ALBERTO A.; SETOH, YIN X.; PERIASAMY, PARTHIBAN; PENG, NIAS Y.; PALI, GABOR; FIGUEIREDO, LUIZ T.; KHROMYKH, ALEXANDER A.; AQUINO, VICTOR H.. Chimeric viruses between Rocio and West Nile: the role for Rocio prM-E proteins in virulence and inhibition of interferon-alpha/beta signaling. SCIENTIFIC REPORTS, v. 7, MAR 20 2017. (14/20930-5, 12/11169-3)