The flaviviruses transmitted by mosquitoes cause a variety of diseases, including fevers, encephalitis and hemorrhagic fevers. The Rocio virus (ROCV) is a member of Japanese encephalitis virus serocomplex and has caused fatal encephalitis in various cities in the São Paulo state-Brazil, between the years 1975-1977. The envelope (E) glycoprotein is the major component of the virion external surface, responsible for neurovirulence of some flaviviruses. The objective of this study is to investigate the role of E protein in viral pathogenesis. Two infectious clones from Brazilian isolates of DENV-3 (D3BR/RP1/2003 and D3BR/SL3/2002) and two chimeric viruses from ROCV were constructed. The E protein from D3BR/RP1/2003 and D3BR/SL3/2002 isolates was replaced by E protein from ROCV using cloning platform in yeast. The viral particles have been recovered after several passages. These constructs could serve as an important tool to study aspects related to pathogenesis, as well as could provide a basis for future vaccines.
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