Analysis of the rpoS status in natural isolates of Escherichia coli

• Grant number: 14/15330-9
• Support Opportunities: Scholarships in Brazil - Master
• Start date: December 01, 2014
• End date: November 30, 2016
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Beny Spira
• Grantee: Jackeline Pinheiro Barros
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Escherichia coli having the ability to survive in diverse habitats, either within the host or the external environment. An important regulator of adaptation of bacteria is the sigma subunit of RNA polymerase responsible for the transcription of most genes associated with the stationary phase and stress situations, such as lack of nutrients, low pH, heat stress, UV exposure and oxidative stress. The rpoS gene has a high degree of polymorphism, easily acquiring or zero attenuation strains grown in the laboratory mutations. This variation gives the bacterium distinct phenotypes relating to nutritional competence and stress resistance. In nature, however, nonfunctional rpoS alleles are apparently less common. To understand the role of rpoS in extra-laboratory conditions, in this study a temporal analysis of rpoS alleles in natural isolates of E. coli will be performed in order to correlate the status of rpoS with the physical and chemical changes that occur over wastewater in the year in São Paulo. (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BARROS, Jackeline Pinheiro. Temporal analysis of the RpoS profile in Escherichia coli isolates from wastewater.. 2017. Master's Dissertation - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.