Biological and physic-chemical evaluations of scaffolds of Chitosan and sodium tripolyphosphate: possible use with mesenchymal cells

• Grant number: 14/18176-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: December 01, 2014
• End date: November 30, 2015
• Field of knowledge: Health Sciences - Pharmacy - Medicines Analysis and Control
• Principal Investigator: Patricia Santos Lopes
• Grantee: Julia Martins Florez
• Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil

Abstract

Tissue regeneration is a process that involves the proliferation of cells within a pre-determined area. In order to create a three-dimensional structure that play such a role, were developed in a previous project (started from the program young talent for science, CAPES) spongy membranes, called scaffold, obtained from Chitosan and sodium tripolyphosphate. Chitosan is a polysaccharide obtained by partial deacetilation of the chitin of structural feature similar to that of glycosaminoglycans. Is a biopolymer which can be obtained at low cost from the shells of some marine animals. Sodium tripolyphosphate performs the function of crosslinker, as this induces a Ionic crosslinking between the amino groups protonated of Chitosan, providing mechanical resistance to the scaffold. Thus, this combination produced from the freeze-drying method will be characterized physically, biologically and physico-chemically, aiming the use as a dressing or as a scaffold for the growth of mesenchymal cells, evaluating the niche theory, that is, how these cells grew in different scaffolds.