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A comparative immunohistochemical study of the inflammatory reaction in specific oral and cutaneous lesions of leprosy

Grant number: 14/16732-3
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): January 01, 2015
Effective date (End): May 31, 2016
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Ana Carolina Fragoso Motta
Grantee:Giovani Antonio Rodrigues
Home Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Leprosy is an ancient chronic infectious disease caused by the Mycobacterium leprae that presents a peculiar tropism for skin and peripheral nerves. The disease is still a global public health problem; Brazil ranks in the second highest number of cases of the disease. The upper airways are considered the most important means of entry and elimination of the bacillus, however lesions in the oral mucosa of patients with leprosy are rare and develop only in advanced stages of the disease. The oral cavity has an important role as a route of transmission of the disease, then it is relevant to study the events associated with the specific oral lesions of leprosy. Considering the importance of Th1, Th2, Tregs and Th17 cells in the leprosy pathogenesis, this work aims to determine the participation of Th1 (IFN-g and TNF-a), Th2 (IL-4 and IL-5), Tregs (IL-10 and TGF-b), Th17 (IL-17) cells and anti-PGL-1 antibody on skin and oral mucosa of patients who developed oral and cutaneous lesions of leprosy. Samples from 15 patients, male and female, who present oral and cutaneous lesions, regardless the clinical form, based on clinical, histopathological and bacteriological criteria will be studied. We intend to determine the phenotypic characteristics of the inflammatory infiltrating cells of oral mucosa and skin through an immunohistochemical analysis, with emphasis to Th1 (IFN-g and TNF-a), Th2 (IL-4 and IL-5), Tregs (IL-10 and TGF-b), Th17 (IL-17) cells and anti-PGL-1 antibody. The ummunoreactivity will be evaluated by independent counts of 10 fields in each sample, and the data will be analyzed by multivariate tests.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
RODRIGUES, G. A.; QUALIO, N. P.; DE MACEDO, L. D.; INNOCENTINI, L. M. A. R.; RIBEIRO-SILVA, A.; FOSS, N. T.; FRADE, M. A. C.; MOTTA, A. C. F. The oral cavity in leprosy: what clinicians need to know. ORAL DISEASES, v. 23, n. 6, p. 749-756, SEP 2017. Web of Science Citations: 1.

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