Influence of synthetic bone biomaterials topography modifications on osteoclast differentiation and activity

• Grant number: 14/22846-1
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: April 01, 2015
• End date: February 18, 2016
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Carlos Alberto de Souza Costa
• Grantee: João Antonio Chaves de Souza
• Supervisor: Vehid Max Salih
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Institution abroad: Plymouth University, England
• Associated to the scholarship: 13/04521-5 - Effects of Yb:YAG laser-modified-titanium surface on osteogenesis. An in vitro study, BP.PD

Abstract

Synthetic biomaterials used as bone substitutes should allow an appropriate bone remodeling with a progressive resorption induced by osteoclasts, followed by bone formation and maturation induced by osteoblasts. Previous in vitro studies demonstrated that changes in the surface topography of beta tricalcium phosphate (bTCP) could influence differentiation and survival of osteoclasts and resorption of these biomaterials. Due to the scarce data concerning the mechanisms by which osteoclasts reabsorb biomaterials with different topographies, our hypothesis is that by changing the surface topography of different bone substitute biomaterials, it could be possible to control the profile of activity and differentiation of osteoclasts. To test this hypothesis, we propose the following two specific objectives:1) Evaluate the capacity of RAW 264.7 to differentiate, survive and resorb different bone substitute biomaterials.2) Assess the signaling pathways activated in these processes. (AU)