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Effect of titanium surface with nanotopography on the interaction between osteoblasts and osteoclasts

Grant number: 17/23888-8
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): March 01, 2019
Effective date (End): January 31, 2022
Field of knowledge:Health Sciences - Dentistry - Oral and Maxillofacial Surgery
Principal Investigator:Márcio Mateus Beloti
Grantee:Rayana Longo Bighetti Trevisan
Home Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Associated scholarship(s):19/09349-2 - Analysis of the whole transcriptome of co-cultured osteoblasts and osteoclasts grown on titanium surface with nanotopography, BE.EP.DR

Abstract

The osseointegration of titanium (Ti) implants is based on bone remodeling, which involves a balance between bone tissue formation by osteoblasts and tissue resorption by osteoclasts. Our group has shown that a Ti surface with nanotopography obtained by chemical conditioning with H2SO4/H2O2 solution is capable of inducing osteoblast differentiation under both osteogenic and non-osteogenic conditions. However, the effect of this surface on differentiation and activity of osteoclasts, and their interaction with osteoblasts have not been investigated yet. In this context, we hypothesized that this nanotopography, in addition to promote osteoblastic differentiation, modulates the osteoblast/osteoclast interaction, inhibiting osteoclastic differentiation. Therefore, the aim of this study is to determine the influence of Ti surface with nanotopography, compared with machined Ti surface (control), on the interaction between osteoblastic and osteoclastic cells. We will evaluate (1) the effect of Ti with nanotopography surface, compared with control Ti surface, on osteoblastic differentiation and osteoclastic formation and activity in isolated cultures of MC3T3-E1 and RAW 264.7 cells, respectively, grown on these surfaces; (2) the effect of MC3T3-E1 cells on osteoclastic formation and activity of RAW 264.7 cells grown on Ti surface with nanotopography, compared with control Ti surface, in indirect co-culture models; (3) the effect of RAW 264.7 cells on osteoblastic differentiation of MC3T3-E1 cells grown on Ti surface with nanotopography, compared with control Ti surface, in indirect co-culture models; (4) the effect of conditioned medium by MC3T3-E1 cells, grown on Ti surface with nanotopography, compared with control Ti surface, on osteoclastic activity and formation of RAW264.7 cells; (5) the effect of conditioned medium by RAW264.7 cells, grown on Ti surface with nanotopography, compared with control Ti surface, on osteoblastic differentiation of MC3T3-E1 cells. The results of this project may contribute to establish new therapeutic approaches, using strategies to modulate osteoblast and osteoclast activities, to favor the interaction between biomaterials and bone tissue. (AU)