Effect of titanium with nanotopography on cell proliferation and osteoclast differentiation

• Grant number: 17/21473-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: January 01, 2018
• End date: December 31, 2018
• Field of knowledge: Health Sciences - Dentistry - Oral and Maxillofacial Surgery
• Principal Investigator: Márcio Mateus Beloti
• Grantee: Beatriz de Camargo Poker
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Osteoclasts are cells responsible for bone resorption, a key event for bone remodeling and consequently for the osseointegration of titanium (Ti) implants. The Ti with nanotopography obtained through the controlled chemical oxidation with H2SO4 and H2O2 induces osteoblast differentiation; however, up to now, there are no data in the literature on the effect of this surface on osteoclasts. Therefore, the aim of the present study is to evaluate the effect of Ti with nanotopography compared with machined Ti (control) on cell proliferation and osteoclast differentiation. The osteoclastic cell line RAW 264.7 will be cultured under osteoclastogenic conditions on Ti discs with nanotopography and Ti control for up to 10 days. At 3, 7 and 10 days, the cell proliferation will be evaluated by MTT colorimetric assay and the osteoclastic differentiation by histochemical labeling for tartrate resistant acid phosphatase (TRAP). The numerical data will be submitted to the test of adherence to the normal distribution to determine the statistical analysis that should be used. The results of our study may guide future investigations on the role of osteoblast/osteoclast interaction in the process of Ti osseointegration. (AU)