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Effect of titanium with nanotopography on cell proliferation and osteoclast differentiation

Grant number: 17/21473-5
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): January 01, 2018
Effective date (End): December 31, 2018
Field of knowledge:Health Sciences - Dentistry - Oral and Maxillofacial Surgery
Principal Investigator:Márcio Mateus Beloti
Grantee:Beatriz de Camargo Poker
Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil


Osteoclasts are cells responsible for bone resorption, a key event for bone remodeling and consequently for the osseointegration of titanium (Ti) implants. The Ti with nanotopography obtained through the controlled chemical oxidation with H2SO4 and H2O2 induces osteoblast differentiation; however, up to now, there are no data in the literature on the effect of this surface on osteoclasts. Therefore, the aim of the present study is to evaluate the effect of Ti with nanotopography compared with machined Ti (control) on cell proliferation and osteoclast differentiation. The osteoclastic cell line RAW 264.7 will be cultured under osteoclastogenic conditions on Ti discs with nanotopography and Ti control for up to 10 days. At 3, 7 and 10 days, the cell proliferation will be evaluated by MTT colorimetric assay and the osteoclastic differentiation by histochemical labeling for tartrate resistant acid phosphatase (TRAP). The numerical data will be submitted to the test of adherence to the normal distribution to determine the statistical analysis that should be used. The results of our study may guide future investigations on the role of osteoblast/osteoclast interaction in the process of Ti osseointegration. (AU)

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