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Long-term evaluation of the stability of dentin matrix following treatment with titanium tetrafluoride and the nano-mechanical properties of the dentin-adhesive interface

Grant number: 14/18848-9
Support Opportunities:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): September 01, 2015
Effective date (End): August 31, 2016
Field of knowledge:Health Sciences - Dentistry - Dental Clinics
Principal Investigator:Roberta Tarkany Basting Höfling
Grantee:Enrico Coser Bridi
Supervisor: Ana Karina Bedran-Russo
Host Institution: Centro de Pesquisas Odontológicas São Leopoldo Mandic. Faculdade São Leopoldo Mandic (SLMANDIC). Sociedade Regional de Ensino e Saúde S/S Ltda (SRES). Campinas , SP, Brazil
Research place: University of Illinois at Chicago (UIC), United States  
Associated to the scholarship:14/05622-2 - In situ evaluation of caries lesions inhibition at the interface tooth/restoration and in vitro evaluation of the longevity of dentin bond after dentin pretreatment with titanium tetrafluoride and self-etching adhesive systems, BP.DR


Objective: The objectives of this study will be to investigate the 1) effects of modification of the dentin matrix by TiF4 on dentin stability and 2) the biomechanical properties of dentin-self-etching adhesive interfaces on the nano-mechanical properties of the adhesive layer, hybrid layer and underlying dentin after long-term storage (up to 6 months). Methods: For first purpose, 15 extracted sound human third molars will be used. Teeth will be ground flat, sectioned into disks in the mesio-distal direction and trimmed to a final rectangular shaped beam. A total of 40 beams will be prepared according to TiF4 application times and storage times. Stability will be assessed immediately, 3 and 6 months following treatment. After, elastic modulus assessment will be performed. For second purpose, 12 extracted sound human third molars will be used. The occlusal surfaces will be ground flat to remove enamel and expose dentin surface. The teeth will be randomly divided into four groups (n=3) according to the restorative procedure. All the samples will be sectioned into 2 beams, and then evaluated at 24 hours, 3 months and 6 months storage. The hardness of dentin, hybrid layer and adhesive layer will be measured using a customized Triboindenter. For Statistical analysis a parametric analysis will be performed. If this procedure is not possible, a non-parametric analysis will be used. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BRIDI, ENRICO COSER; LEME-KRAUS, ARIENE ARCAS; BASTING, ROBERTA TARKANY; BEDRAN-RUSSO, ANA KARINA. Long-term nanomechanical properties and gelatinolytic activity of titanium tetrafluoride-treated adhesive dentin interface. Dental Materials, v. 35, n. 10, p. 1471-1478, . (14/18848-9)

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